TY - JOUR
T1 - ER exit sites are physical and functional core autophagosome biogenesis components
AU - Graef, Martin
AU - Friedman, Jonathan R.
AU - Graham, Christopher
AU - Babu, Mohan
AU - Nunnari, Jodi
PY - 2013/9/15
Y1 - 2013/9/15
N2 - Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outstanding questions. Here we show, using proteomic, cytological, and functional analyses, that autophagosomes are spatially, physically, and functionally linked to endoplasmic reticulum exit sites (ERES), which are specialized regions of the endoplasmic reticulum where COPII transport vesicles are generated. Our data demonstrate that ERES are core autophagosomal biogenesis components whose function is required for the hierarchical assembly of the autophagy machinery immediately downstream of the Atg1 kinase complex at phagophore assembly sites.
AB - Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outstanding questions. Here we show, using proteomic, cytological, and functional analyses, that autophagosomes are spatially, physically, and functionally linked to endoplasmic reticulum exit sites (ERES), which are specialized regions of the endoplasmic reticulum where COPII transport vesicles are generated. Our data demonstrate that ERES are core autophagosomal biogenesis components whose function is required for the hierarchical assembly of the autophagy machinery immediately downstream of the Atg1 kinase complex at phagophore assembly sites.
UR - http://www.scopus.com/inward/record.url?scp=84884487128&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884487128&partnerID=8YFLogxK
U2 - 10.1091/mbc.E13-07-0381
DO - 10.1091/mbc.E13-07-0381
M3 - Article
C2 - 23904270
AN - SCOPUS:84884487128
SN - 1059-1524
VL - 24
SP - 2918
EP - 2931
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 18
ER -