Abstract
The ability of DNA oligonucleotides, neutral peptide nucleic acids (PNAs), and oligonucleotide conjugates to hybridize to inverted repeat sequences within supercolled double-stranded DNA by Watson-Crick base-pairing is examined. PNAs and oligonucleotide conjugates initiate and maintain strand invasion under more stringent conditions than do unmodified DNA oligonucleotides. PNAs hybridize rapidly and, once bound, hold open a target site allowing oligonucleotides to base-pair to the displaced strand under conditions that would otherwise preclude hybridization. The ability to manipulate hybridization efficiency through different options for the alteration of oligomer charge should have important implications for optimizing sequence-specific recognition of DNA.
Original language | English (US) |
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Pages (from-to) | 1700-1704 |
Number of pages | 5 |
Journal | Nature biotechnology |
Volume | 14 |
Issue number | 13 |
DOIs | |
State | Published - Dec 1996 |
Keywords
- Macromolecular engineering
- Oligonucleotide
- Peptide nucleic acid
- Strand invasion
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Molecular Medicine
- Biomedical Engineering