Energy transfer chemiluminescence for ratiometric pH imaging

Weiwei An, Ralph P. Mason, Alexander R. Lippert

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Chemiluminescence imaging offers a low background and high sensitivity approach to imaging analytes in living cells and animals. Intensity-based measurements have been developed, but require careful consideration of kinetics, probe localization, and fluctuations in quantum yield, all of which complicate quantification. Here, we report a ratiometric strategy for quantitative chemiluminescence imaging of pH. The strategy relies on an energy transfer cascade of chemiluminescence emission from a spiroadamantane 1,2-dioxetane to a ratiometric pH indicator via fluorescent dyes in Enhancer solutions. Monitoring the pH-dependent changes in chemiluminescence emission at multiple wavelengths enables ratiometric imaging and quantification of pH independent from variations due to kinetics and probe concentration.

Original languageEnglish (US)
Pages (from-to)4176-4182
Number of pages7
JournalOrganic and Biomolecular Chemistry
Issue number22
StatePublished - 2018

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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