Endothelin-1 Stimulates Leptin Production in Adipocytes

Yumei Xiong, Hirokazu Tanaka, James A Richardson, S. Clay Williams, Clive A. Slaughter, Motonao Nakamura, Jin Long Chen, Masashi Yanagisawa

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


Leptin is an adipocyte-derived hormone that regulates body fat stores and feeding behavior. In an effort to identify endogenous diffusible modulators of leptin production, we found that endothelin-1 (ET-1) up-regulates leptin expression in adipocytes. ET-1 is as potent and efficacious as insulin in stimulating leptin production in two different adipocyte cell lines. Endothelins stimulate leptin production via the endothelin-A receptor (ET A), as judged by a potency rank order of ET-1 ≫ ET-3. We detected expression of ETA but not ETB in both cell lines by Northern blot analysis. In addition, the ETA-selective antagonist FR139317 inhibited ET-1-induced leptin expression more potently than did the ETB-selective antagonist BQ788. ET-1 and insulin positively interact with each other in increasing leptin production in adipocytes. In primary mouse white fat cells, we detected expression of both ETA and ET B by Northern blot and in situ hybridization analyses. We conclude that ET-1 stimulates leptin production via the ETA receptor in cultured adipocytes.

Original languageEnglish (US)
Pages (from-to)28471-28477
Number of pages7
JournalJournal of Biological Chemistry
Issue number30
StatePublished - Jul 27 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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