Endothelin-1 in luteal tissue

S. Usuki, N. Suzuki, H. Matsumoto, M. Yanagisawa, T. Masaki

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


The aim of this study was to immunologically and biologically detect endothelin-1 (ET-1) in rat corpora lutea (CL). Recently, we established a highly sensitive and specific sandwich-enzyme immunoassay (EIA) for ET-1. Using this assay, the presence of ET-1 was investigated in superovulated ovaries, induced with pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG), and ovaries from pseudopregnant rats, induced by cervical stimulation. A high concentration of immunoreactive endothelin-1 (ir-ET-1) was found in the CL. On reverse phase-high performance liquid chromatography (RP-HPLC) coupled with EIA, ir-ET-1 was exclusively eluted at the same position as synthetic ET-1, indicating that ir-ET-1 is identical to ET-1. The level of ir-ET-1 was significantly (P < 0.001) higher in the CL 7 days after hCG injection than it was 4 days after hCG injection. On day 7 of pseudopregnancy (PSP), the ir-ET-1 level was also significantly (P < 0.001) higher than on day 4 of PSP. These results demonstrated that ET-1 is present in a high concentration in the CL, suggesting a new intraovarian peptide which may have a physiological function in the ovary and which may vary in quantity according to the age of the CL.

Original languageEnglish (US)
Pages (from-to)147-151
Number of pages5
JournalMolecular and Cellular Endocrinology
Issue number1-3
StatePublished - Sep 1991


  • Corpus luteum
  • Endothelin-1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology


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