TY - JOUR
T1 - Endothelial microparticles and the antiangiogenic state in preeclampsia and the postpartum period
AU - Petrozella, Loren
AU - Mahendroo, Mala
AU - Timmons, Brenda
AU - Roberts, Scott
AU - McIntire, Donald
AU - Alexander, James M.
N1 - Funding Information:
Supported by National Institutes of Health/National Center for Research Resources Grant 5 UL1 RR024982-03 , Milton Packer, MD (principal investigator).
PY - 2012/8
Y1 - 2012/8
N2 - Objective: We sought to determine if endothelial microparticles (EMPs), markers of endothelial damage, are associated with soluble fms-like tyrosine kinase 1 (sFlt1), soluble endoglin, and placental growth factor (PlGF) in women with preeclampsia. Study Design: A prospective cohort study was conducted on 20 preeclamptic women and 20 controls. EMPs by flow cytometry, sFlt1, soluble endoglin, and PlGF were measured at time of enrollment, 48-hours postpartum, and 1-week postpartum. Results: Preeclamptic CD31+/42-, CD62E+, and CD105+ EMP levels were significantly elevated in preeclamptics vs controls at time of enrollment. The sFlt1:PlGF ratio was correlated with CD31+/42- and CD105+ EMPs (r = 0.69 and r = 0.51, respectively) in preeclampsia. Levels of CD31 +/42- EMPs remained elevated 1-week postpartum (P =.026). Conclusion: EMPs are elevated in preeclampsia. The correlation of EMPs and the sFlt1:PlGF ratio suggests that antiangiogenesis is related to apoptosis of the endothelia. Endothelial damage persists 1 week after delivery.
AB - Objective: We sought to determine if endothelial microparticles (EMPs), markers of endothelial damage, are associated with soluble fms-like tyrosine kinase 1 (sFlt1), soluble endoglin, and placental growth factor (PlGF) in women with preeclampsia. Study Design: A prospective cohort study was conducted on 20 preeclamptic women and 20 controls. EMPs by flow cytometry, sFlt1, soluble endoglin, and PlGF were measured at time of enrollment, 48-hours postpartum, and 1-week postpartum. Results: Preeclamptic CD31+/42-, CD62E+, and CD105+ EMP levels were significantly elevated in preeclamptics vs controls at time of enrollment. The sFlt1:PlGF ratio was correlated with CD31+/42- and CD105+ EMPs (r = 0.69 and r = 0.51, respectively) in preeclampsia. Levels of CD31 +/42- EMPs remained elevated 1-week postpartum (P =.026). Conclusion: EMPs are elevated in preeclampsia. The correlation of EMPs and the sFlt1:PlGF ratio suggests that antiangiogenesis is related to apoptosis of the endothelia. Endothelial damage persists 1 week after delivery.
KW - endothelial microparticles
KW - preeclampsia
KW - pregnancy
KW - soluble fms-like tyrosine kinase 1:placental growth factor ratio
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U2 - 10.1016/j.ajog.2012.06.011
DO - 10.1016/j.ajog.2012.06.011
M3 - Article
C2 - 22840727
AN - SCOPUS:84864408404
SN - 0002-9378
VL - 207
SP - 140.e20-140.e26
JO - American journal of obstetrics and gynecology
JF - American journal of obstetrics and gynecology
IS - 2
ER -