Endothelial and Leptin Receptor+ cells promote the maintenance of stem cells and hematopoiesis in early postnatal murine bone marrow

Nergis Kara, Yuanyuan Xue, Zhiyu Zhao, Malea M. Murphy, Stefano Comazzetto, Ashley Lesser, Liming Du, Sean J. Morrison

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Mammalian hematopoietic stem cells (HSCs) colonize the bone marrow during late fetal development, and this becomes the major site of hematopoiesis after birth. However, little is known about the early postnatal bone marrow niche. We performed single-cell RNA sequencing of mouse bone marrow stromal cells at 4 days, 14 days, and 8 weeks after birth. Leptin-receptor-expressing (LepR+) stromal cells and endothelial cells increased in frequency during this period and changed their properties. At all postnatal stages, LepR+ cells and endothelial cells expressed the highest stem cell factor (Scf) levels in the bone marrow. LepR+ cells expressed the highest Cxcl12 levels. In early postnatal bone marrow, SCF from LepR+/Prx1+ stromal cells promoted myeloid and erythroid progenitor maintenance, while SCF from endothelial cells promoted HSC maintenance. Membrane-bound SCF in endothelial cells contributed to HSC maintenance. LepR+ cells and endothelial cells are thus important niche components in early postnatal bone marrow.

Original languageEnglish (US)
Pages (from-to)348-360.e6
JournalDevelopmental cell
Volume58
Issue number5
DOIs
StatePublished - Mar 13 2023

Keywords

  • erythropoiesis
  • myelopoiesis
  • niche
  • stem cell factor
  • stromal cells

ASJC Scopus subject areas

  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology
  • Developmental Biology
  • Cell Biology

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