Endogenous inhibitor of GABA binding in mammalian brain

Donald V. Greenlee; Paul C. van Ness; Richard W. Olsen

doi:10.1016/0024-3205(78)90062-0

Endogenous inhibitor of GABA binding in mammalian brain

Donald V. Greenlee, Paul C. van Ness, Richard W. Olsen

Neurology & Neurotherapeutics

Research output: Contribution to journal › Article › peer-review

105 Scopus citations

Abstract

Binding of radioactive gamma-aminobutyric acid to homogenates of mammalian brain was detected by a centrifugation assay. The binding capacity of the tissue was maximal and stable with time only if the tissue was thoroughly washed to remove an endogenous inhibitor of binding. With such washed tissue, binding to total rat or cow brain appeared to involve two populations of sites in the absence of sodium ions, the major site having a dissociation constant of 150 nM and saturating at 80 pmol/g brain, and a minor site with a K_D of 20 nM and saturating at 20 pmol/g wet tissue. This sodium-independent GABA binding as a whole was localized in the crude mitochondrial, microsomal, and synaptosomal membrane fractions.

Original language	English (US)
Pages (from-to)	1653-1662
Number of pages	10
Journal	Life Sciences
Volume	22
Issue number	18
DOIs	https://doi.org/10.1016/0024-3205(78)90062-0
State	Published - May 8 1978

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(78)90062-0

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title = "Endogenous inhibitor of GABA binding in mammalian brain",

abstract = "Binding of radioactive gamma-aminobutyric acid to homogenates of mammalian brain was detected by a centrifugation assay. The binding capacity of the tissue was maximal and stable with time only if the tissue was thoroughly washed to remove an endogenous inhibitor of binding. With such washed tissue, binding to total rat or cow brain appeared to involve two populations of sites in the absence of sodium ions, the major site having a dissociation constant of 150 nM and saturating at 80 pmol/g brain, and a minor site with a KD of 20 nM and saturating at 20 pmol/g wet tissue. This sodium-independent GABA binding as a whole was localized in the crude mitochondrial, microsomal, and synaptosomal membrane fractions.",

author = "Greenlee, {Donald V.} and {van Ness}, {Paul C.} and Olsen, {Richard W.}",

note = "Funding Information: Acknowledgments : We thank Dra . W . B . LEVY, B . MEINERS, and M . R . TICKU for helpful discussion. Supported by NSF Grant BNS 73-02078 and NIH Grants NS 12422 and RCDA NS 00224 to R . W . OLSEN . R . W . OLSEN is an Alfred P . Sloan Fellow .",

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doi = "10.1016/0024-3205(78)90062-0",

language = "English (US)",

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TY - JOUR

T1 - Endogenous inhibitor of GABA binding in mammalian brain

AU - Greenlee, Donald V.

AU - van Ness, Paul C.

AU - Olsen, Richard W.

N1 - Funding Information: Acknowledgments : We thank Dra . W . B . LEVY, B . MEINERS, and M . R . TICKU for helpful discussion. Supported by NSF Grant BNS 73-02078 and NIH Grants NS 12422 and RCDA NS 00224 to R . W . OLSEN . R . W . OLSEN is an Alfred P . Sloan Fellow .

PY - 1978/5/8

Y1 - 1978/5/8

N2 - Binding of radioactive gamma-aminobutyric acid to homogenates of mammalian brain was detected by a centrifugation assay. The binding capacity of the tissue was maximal and stable with time only if the tissue was thoroughly washed to remove an endogenous inhibitor of binding. With such washed tissue, binding to total rat or cow brain appeared to involve two populations of sites in the absence of sodium ions, the major site having a dissociation constant of 150 nM and saturating at 80 pmol/g brain, and a minor site with a KD of 20 nM and saturating at 20 pmol/g wet tissue. This sodium-independent GABA binding as a whole was localized in the crude mitochondrial, microsomal, and synaptosomal membrane fractions.

AB - Binding of radioactive gamma-aminobutyric acid to homogenates of mammalian brain was detected by a centrifugation assay. The binding capacity of the tissue was maximal and stable with time only if the tissue was thoroughly washed to remove an endogenous inhibitor of binding. With such washed tissue, binding to total rat or cow brain appeared to involve two populations of sites in the absence of sodium ions, the major site having a dissociation constant of 150 nM and saturating at 80 pmol/g brain, and a minor site with a KD of 20 nM and saturating at 20 pmol/g wet tissue. This sodium-independent GABA binding as a whole was localized in the crude mitochondrial, microsomal, and synaptosomal membrane fractions.

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VL - 22

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JO - Life Sciences

JF - Life Sciences

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ER -

Endogenous inhibitor of GABA binding in mammalian brain

Abstract

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