@article{cc8fb55d781649378ac645e96d381658,
title = "Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton",
abstract = "Ena/VASP proteins and the WAVE regulatory complex (WRC) regulate cell motility by virtue of their ability to independently promote actin polymerization. We demonstrate that Ena/VASP and the WRC control actin polymerization in a cooperative manner through the interaction of the Ena/VASP EVH1 domain with an extended proline rich motif in Abi. This interaction increases cell migration and enables VASP to cooperatively enhance WRC stimulation of Arp2/3 complex-mediated actin assembly invitro inthe presence of Rac. Loss of this interaction in Drosophila macrophages results in defects in lamellipodia formation, cell spreading, and redistribution of Ena to the tips of filopodia-like extensions. Rescue experiments of abi mutants also reveals a physiological requirement for the Abi:Ena interaction in photoreceptor axon targeting and oogenesis. Our data demonstrate that the activities of Ena/VASP and the WRC are intimately linked to ensure optimal control of actin polymerization during cell migration and development.",
author = "Chen, {Xing Judy} and Squarr, {Anna Julia} and Raiko Stephan and Baoyu Chen and Higgins, {Theresa E.} and Barry, {David J.} and Martin, {Morag C.} and Rosen, {Michael K.} and Sven Bogdan and Michael Way",
note = "Funding Information: We thank Theresia Stradal (Helmholtz Centre for Infection Research) and Frank Gertler (MIT) for providing antibodies against human WAVE components and MV D7 cells, respectively. We also thank Laura Machesky (Beatson Institute), Theresia Stradal, and Shiro Suetsugu (Nara Institute of Science and Technology) for WAVE1, WAVE2, and WAVE3 clones, respectively. We also thank the London Research Institute Protein Analysis & Proteomics, Peptide Synthesis, Light Microscopy services, Nic Tapon, and Barry Thompson laboratories for technical support and reagents, as well as members of the Way laboratory, Yanxiang Zhou, and Helen Walden for comments on the manuscript. This work was supported by Cancer Research UK (X.J.C., D.J.B., M.C.M., and M.W.); the Deutsche Forschungsgemeinschaft (DFG) and the Cells-in-Motion Cluster of Excellence (EXC 1003 – CiM), University of M{\"u}nster (A.J.S., R.S., and S.B.); the Howard Hughes Medical Institute; and grants from the NIH (R01-GM56322) and Welch Foundation (I–1544; to B.C. and M.K.R.). Publisher Copyright: {\textcopyright} 2014 The Authors.",
year = "2014",
month = sep,
day = "8",
doi = "10.1016/j.devcel.2014.08.001",
language = "English (US)",
volume = "30",
pages = "569--584",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "5",
}