@article{ab6c6abd8f1047478059c4e3cef83d61,
title = "Elongin functions as a loading factor for Mediator at ATF6α-regulated ER stress response genes",
abstract = "The bZIP transcription factor ATF6α is a master regulator of endoplasmic reticulum (ER) stress response genes. In this report, we identify the multifunctional RNA polymerase II transcription factor Elongin as a cofactor for ATF6α-dependent transcription activation. Biochemical studies reveal that Elongin functions at least in part by facilitating ATF6α-dependent loading of Mediator at the promoters and enhancers of ER stress response genes. Depletion of Elongin from cells leads to impaired transcription of ER stress response genes and to defects in the recruitment of Mediator and its CDK8 kinase subunit. Taken together, these findings bring to light a role for Elongin as a loading factor for Mediator during the ER stress response.",
keywords = "Enhancer, Mediator, RNA polymerase II, Transcription",
author = "Yanfeng He and Shigeo Sato and Chieri Tomomori-Sato and Shiyuan Chen and Goode, {Zach H.} and Conaway, {Joan W.} and Conaway, {Ronald C.}",
note = "Funding Information: ACKNOWLEDGMENTS. We are grateful to colleagues in the Stowers Institute Sequencing and Tissue Culture Cores for essential contributions and to members of the J.W.C. and R.C.C. laboratory for helpful discussions. We acknowledge the University of Kansas Medical Center{\textquoteright}s Genomics Core for generating data on the Illumina NovaSeq 6000 System. The Genomics Core is supported by the Kansas Intellectual and Developmental Disabilities Research Center (NIH U54 HD 090216), Molecular Regulation of Cell Development and Differentiation—COBRE (P30 GM122731-03), and an NIH S10 High-End Instrumentation Grant (NIH S10OD021743). Work in the J.W.C. and R.C.C. laboratory is supported by funds from the Stowers Institute for Medical Research and a grant to the Stowers Institute from the Helen Nelson Medical Research Fund at the Greater Kansas City Community Foundation. Publisher Copyright: {\textcopyright} 2021 National Academy of Sciences. All rights reserved.",
year = "2021",
month = sep,
day = "28",
doi = "10.1073/pnas.2108751118",
language = "English (US)",
volume = "118",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "39",
}