Electron capture dissociation for robust production of sequence tags from eukaryotic proteins

Neil L. Kelleher; Steven Patrie; Jeffrey R. Johnson; Fanyu Meng; Andrew J. Forbes

Electron capture dissociation for robust production of sequence tags from eukaryotic proteins

Neil L. Kelleher, Steven Patrie, Jeffrey R. Johnson, Fanyu Meng, Andrew J. Forbes

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The potential of electron capture dissociation (ECD) was translated into identification of heavily-modified proteins from eukaryotic proteins isolated from S. cerevisiae. Yeast cells were grown to stationary phase and collected by centrifugation. Cell lysis by sonication was followed by fractionation of the cell extract using a combination of continuous-elution PAGE and reversed-phase HPLC. It was found that ∼40-50% of fragment ions from standard threshold dissociation techniques do not occur in strings, for ions of ∼75 wild-type proteins.

Original language	English (US)
Title of host publication	Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	101-102
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

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title = "Electron capture dissociation for robust production of sequence tags from eukaryotic proteins",

abstract = "The potential of electron capture dissociation (ECD) was translated into identification of heavily-modified proteins from eukaryotic proteins isolated from S. cerevisiae. Yeast cells were grown to stationary phase and collected by centrifugation. Cell lysis by sonication was followed by fractionation of the cell extract using a combination of continuous-elution PAGE and reversed-phase HPLC. It was found that ∼40-50% of fragment ions from standard threshold dissociation techniques do not occur in strings, for ions of ∼75 wild-type proteins.",

author = "Kelleher, {Neil L.} and Steven Patrie and Johnson, {Jeffrey R.} and Fanyu Meng and Forbes, {Andrew J.}",

year = "2002",

language = "English (US)",

pages = "101--102",

booktitle = "Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - GEN

T1 - Electron capture dissociation for robust production of sequence tags from eukaryotic proteins

AU - Kelleher, Neil L.

AU - Patrie, Steven

AU - Johnson, Jeffrey R.

AU - Meng, Fanyu

AU - Forbes, Andrew J.

PY - 2002

Y1 - 2002

N2 - The potential of electron capture dissociation (ECD) was translated into identification of heavily-modified proteins from eukaryotic proteins isolated from S. cerevisiae. Yeast cells were grown to stationary phase and collected by centrifugation. Cell lysis by sonication was followed by fractionation of the cell extract using a combination of continuous-elution PAGE and reversed-phase HPLC. It was found that ∼40-50% of fragment ions from standard threshold dissociation techniques do not occur in strings, for ions of ∼75 wild-type proteins.

AB - The potential of electron capture dissociation (ECD) was translated into identification of heavily-modified proteins from eukaryotic proteins isolated from S. cerevisiae. Yeast cells were grown to stationary phase and collected by centrifugation. Cell lysis by sonication was followed by fractionation of the cell extract using a combination of continuous-elution PAGE and reversed-phase HPLC. It was found that ∼40-50% of fragment ions from standard threshold dissociation techniques do not occur in strings, for ions of ∼75 wild-type proteins.

UR - http://www.scopus.com/inward/record.url?scp=2442649076&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442649076&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442649076

SP - 101

EP - 102

BT - Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Electron capture dissociation for robust production of sequence tags from eukaryotic proteins

Abstract

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ASJC Scopus subject areas

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