TY - JOUR
T1 - Effects of evodiamine on the secretion of testosterone in rat testicular interstitial cells
AU - Lin, Ho
AU - Tsai, Shiow Chwen
AU - Chen, Jiann Jong
AU - Chiao, Yu Chung
AU - Wang, Shyi Wu
AU - Wang, Guei Jane
AU - Chen, Chieh Fu
AU - Wang, Paulus S.
N1 - Funding Information:
From the Department of Physiology, School of Life Science, and School of Medicine, National Yang-Ming University, Taipei; Department of Physiology, Chang Gung University, Taoyuan; and National Research Institute of Chinese Medicine, Taipei, Taiwan, Republic of China. Submitted February 1, 1999; accepted June 29, 1999. Supported by Grant No. NRICM-87109 from the National Research Institute of Chinese Medicine, and awards from the Medical Research and Advancement Foundation in memory of Dr Chi-Shuen Tsou, Republic of China, (to P.S. W.). Address reprint requests to Paulus S. Wang, PhD, Department of Physiology, School of Life Science, National Yang-Ming University, Shih-Pai, Taipei 11221, Taiwan, Republic of China. Copyright © 1999 by W.B. Saunders Company 0026-0495/99/4812-0014510.00/0
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1999
Y1 - 1999
N2 - Evodiamine, a bioactive component isolated from the Chinese medicine Wu- chu-yu, exhibits vasodilative and antianoxic action. Although evodiamine indeed has many biological effects, its effects on the endocrine system are not clear. The present study explored the effects of evodiamine on testosterone secretion in vitro. Rat collagenase-dispersed testicular interstitial cells (TICs) were incubated with evodiamine (0 to 10-4 mol/L) in the presence or absence of human chorionic gonadotropin (hCG), forskolin, 8-bromo-adenosine 3':5'-cyclic monophosphate (8-Br-cAMP), or steroidogenic precursors (including 25hydroxycholesterol, pregnenolone, progesterone, 17α- hydroxyprogesterone, and androstenedione) at 34°C for 1 hour. The testosterone concentration in the media samples was measured by radioimmunoassay. Evodiamine 10-4 mol/L was effective to reduce both basal and hCG-stimulated testosterone secretion in rat TICs after 1,2, or 4 hours of incubation. The stimulatory effect of forskolin on testosterone release in TICs was prevented by administration of evodiamine. Evodiamine 10-4 mol/L also decreased 8-Br-cAMP- and androstenedione-stimulated testosterone secretion. These results suggest that evodiamine reduces testosterone secretion in rat TICs via a mechanism involving reduced activity of cAMP- related pathways and 17β-hydroxysteroid dehydrogenase (17β-HSD).
AB - Evodiamine, a bioactive component isolated from the Chinese medicine Wu- chu-yu, exhibits vasodilative and antianoxic action. Although evodiamine indeed has many biological effects, its effects on the endocrine system are not clear. The present study explored the effects of evodiamine on testosterone secretion in vitro. Rat collagenase-dispersed testicular interstitial cells (TICs) were incubated with evodiamine (0 to 10-4 mol/L) in the presence or absence of human chorionic gonadotropin (hCG), forskolin, 8-bromo-adenosine 3':5'-cyclic monophosphate (8-Br-cAMP), or steroidogenic precursors (including 25hydroxycholesterol, pregnenolone, progesterone, 17α- hydroxyprogesterone, and androstenedione) at 34°C for 1 hour. The testosterone concentration in the media samples was measured by radioimmunoassay. Evodiamine 10-4 mol/L was effective to reduce both basal and hCG-stimulated testosterone secretion in rat TICs after 1,2, or 4 hours of incubation. The stimulatory effect of forskolin on testosterone release in TICs was prevented by administration of evodiamine. Evodiamine 10-4 mol/L also decreased 8-Br-cAMP- and androstenedione-stimulated testosterone secretion. These results suggest that evodiamine reduces testosterone secretion in rat TICs via a mechanism involving reduced activity of cAMP- related pathways and 17β-hydroxysteroid dehydrogenase (17β-HSD).
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U2 - 10.1016/S0026-0495(99)90241-X
DO - 10.1016/S0026-0495(99)90241-X
M3 - Article
C2 - 10599984
AN - SCOPUS:0032715166
SN - 0026-0495
VL - 48
SP - 1532
EP - 1535
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
IS - 12
ER -