Effects of Danazol on endometrial DNA synthesis in rats

Satoe Suzuki; Shinobu Sakamoto; Hideki Kudo; Shuji Sassa; Yoshie Sugiura; Katsuhiko Kuwa; Noriyuki Kasahara; Takao Mori; Hiroshi Nagasawa

doi:10.1016/0039-128X(93)90034-K

Effects of Danazol on endometrial DNA synthesis in rats

Satoe Suzuki, Shinobu Sakamoto, Hideki Kudo, Shuji Sassa, Yoshie Sugiura, Katsuhiko Kuwa, Noriyuki Kasahara, Takao Mori, Hiroshi Nagasawa

Neuroscience

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The suppressive effects of Danazol, an isoxazol derivative of the synthetic steroid 17 α-ethinyltestosterone, on endometrial DNA synthesis were investigated in rats by immunohistochemistry with bromodeoxyuridine (BrdU) and DNA-synthesizing enzyme assays. Rats treated with Danazol for 14 days at 17-19 weeks of age showed a decrease of plasma gonadotropins associated with ovarian hypofunction, persistent diestrus, and a smaller number of corpora lutea in ovary, resulting in no BrdU-immunoreactive (S-phase) cells in endometrial epithelium and lower activity of thymidine kinase in the uterus compared with control.

Original language	English (US)
Pages (from-to)	551-553
Number of pages	3
Journal	Steroids
Volume	58
Issue number	11
DOIs	https://doi.org/10.1016/0039-128X(93)90034-K
State	Published - Nov 1993

Keywords

DNA synthesis
Danazol
endometrium

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

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10.1016/0039-128X(93)90034-K

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title = "Effects of Danazol on endometrial DNA synthesis in rats",

abstract = "The suppressive effects of Danazol, an isoxazol derivative of the synthetic steroid 17 α-ethinyltestosterone, on endometrial DNA synthesis were investigated in rats by immunohistochemistry with bromodeoxyuridine (BrdU) and DNA-synthesizing enzyme assays. Rats treated with Danazol for 14 days at 17-19 weeks of age showed a decrease of plasma gonadotropins associated with ovarian hypofunction, persistent diestrus, and a smaller number of corpora lutea in ovary, resulting in no BrdU-immunoreactive (S-phase) cells in endometrial epithelium and lower activity of thymidine kinase in the uterus compared with control.",

keywords = "DNA synthesis, Danazol, endometrium",

author = "Satoe Suzuki and Shinobu Sakamoto and Hideki Kudo and Shuji Sassa and Yoshie Sugiura and Katsuhiko Kuwa and Noriyuki Kasahara and Takao Mori and Hiroshi Nagasawa",

note = "Funding Information: Acknowledgments This work was supported in part by Grants-in-Aid for Fundamental Scientific Research from the Ministry of Education, Science and Culture, Japant o S. Sakamoto. We thank Mr. Masaru Tajima for his excellent technical assistance.",

year = "1993",

month = nov,

doi = "10.1016/0039-128X(93)90034-K",

language = "English (US)",

volume = "58",

pages = "551--553",

journal = "Steroids",

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number = "11",

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T1 - Effects of Danazol on endometrial DNA synthesis in rats

AU - Suzuki, Satoe

AU - Sakamoto, Shinobu

AU - Kudo, Hideki

AU - Sassa, Shuji

AU - Sugiura, Yoshie

AU - Kuwa, Katsuhiko

AU - Kasahara, Noriyuki

AU - Mori, Takao

AU - Nagasawa, Hiroshi

N1 - Funding Information: Acknowledgments This work was supported in part by Grants-in-Aid for Fundamental Scientific Research from the Ministry of Education, Science and Culture, Japant o S. Sakamoto. We thank Mr. Masaru Tajima for his excellent technical assistance.

PY - 1993/11

Y1 - 1993/11

N2 - The suppressive effects of Danazol, an isoxazol derivative of the synthetic steroid 17 α-ethinyltestosterone, on endometrial DNA synthesis were investigated in rats by immunohistochemistry with bromodeoxyuridine (BrdU) and DNA-synthesizing enzyme assays. Rats treated with Danazol for 14 days at 17-19 weeks of age showed a decrease of plasma gonadotropins associated with ovarian hypofunction, persistent diestrus, and a smaller number of corpora lutea in ovary, resulting in no BrdU-immunoreactive (S-phase) cells in endometrial epithelium and lower activity of thymidine kinase in the uterus compared with control.

AB - The suppressive effects of Danazol, an isoxazol derivative of the synthetic steroid 17 α-ethinyltestosterone, on endometrial DNA synthesis were investigated in rats by immunohistochemistry with bromodeoxyuridine (BrdU) and DNA-synthesizing enzyme assays. Rats treated with Danazol for 14 days at 17-19 weeks of age showed a decrease of plasma gonadotropins associated with ovarian hypofunction, persistent diestrus, and a smaller number of corpora lutea in ovary, resulting in no BrdU-immunoreactive (S-phase) cells in endometrial epithelium and lower activity of thymidine kinase in the uterus compared with control.

KW - DNA synthesis

KW - Danazol

KW - endometrium

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DO - 10.1016/0039-128X(93)90034-K

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VL - 58

SP - 551

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JO - Steroids

JF - Steroids

IS - 11

ER -

Effects of Danazol on endometrial DNA synthesis in rats

Abstract

Keywords

ASJC Scopus subject areas

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