Dynein-driven mitotic spindle positioning restricted to anaphase by she1p inhibition of dynactin recruitment

Jeffrey B. Woodruff, David G. Drubin, Georjana Barnes

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Dynein is a minus-end-directed microtubule motor important for mitotic spindle positioning. In budding yeast, dynein activity is restricted to anaphase when the nucleus enters the bud neck, yet the nature of the underlying regulatory mechanism is not known. Here, the microtubule-associated protein She1p is identified as a novel regulator of dynein activity. In she1Δ cells, dynein is activated throughout the cell cycle, resulting in aberrant spindle movements that misposition the spindle. We also found that dynactin, a cofactor essential for dynein motor function, is a dynamic complex whose recruitment to astral microtubules (aMTs) increases dramatically during anaphase. Interestingly, loss of She1p eliminates the cell-cycle regulation of dynactin recruitment and permits enhanced dynactin accumulation on aMTs throughout the cell cycle. Furthermore, localization of the dynactin complex to aMTs requires dynein, suggesting that dynactin is recruited to aMTs via interaction with dynein and not the microtubule itself. Lastly, we present evidence supporting the existence of an incomplete dynactin subcomplex localized at the SPB, and a complete complex that is loaded onto aMTs from the cytoplasm. We propose that She1p restricts dynein-dependent spindle positioning to anaphase by inhibiting the association of dynein with the complete dynactin complex.

Original languageEnglish (US)
Pages (from-to)3003-3011
Number of pages9
JournalMolecular biology of the cell
Issue number13
StatePublished - Jul 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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