TY - JOUR
T1 - Dynamic Protein-RNA recognition in primary MicroRNA processing
AU - Ruiz-Arroyo, Victor M.
AU - Nam, Yunsun
N1 - Funding Information:
The goal of the review was to highlight the recent advances in the last two years. We apologize to authors whose work was not explicitly included due to space limitations. This work was supported by the National Institutes of Health ( R01GM122960 ) and the Welch Foundation ( I-2115-20220331 ). Y.N. is a Packard Fellow, Pew Scholar, and Southwestern Medical Foundation Scholar in Biomedical Research.
Funding Information:
The goal of the review was to highlight the recent advances in the last two years. We apologize to authors whose work was not explicitly included due to space limitations. This work was supported by the National Institutes of Health (R01GM122960) and the Welch Foundation (I-2115-20220331). Y.N. is a Packard Fellow, Pew Scholar, and Southwestern Medical Foundation Scholar in Biomedical Research.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/10
Y1 - 2022/10
N2 - MicroRNAs are prevalent regulators of gene expression, controlling most of the proteome in multicellular organisms. To generate the functional small RNAs, precise processing steps are required. In animals, microRNA biogenesis is initiated by Microprocessor that minimally consists of the Drosha enzyme and its partner, DGCR8. This first step is critical for selecting primary microRNAs, and many RNA-binding proteins and regulatory pathways target both the accuracy and efficiency of microRNA maturation. Structures of Drosha and DGCR8 in complex with primary microRNAs elucidate how RNA structural features rather than sequence provide the framework for substrate recognition. Comparing multiple states of Microprocessor and the closely related Dicer homologs shed light on the dynamic protein-RNA complex assembly and disassembly required to recognize RNAs with diverse sequences via common structural features.
AB - MicroRNAs are prevalent regulators of gene expression, controlling most of the proteome in multicellular organisms. To generate the functional small RNAs, precise processing steps are required. In animals, microRNA biogenesis is initiated by Microprocessor that minimally consists of the Drosha enzyme and its partner, DGCR8. This first step is critical for selecting primary microRNAs, and many RNA-binding proteins and regulatory pathways target both the accuracy and efficiency of microRNA maturation. Structures of Drosha and DGCR8 in complex with primary microRNAs elucidate how RNA structural features rather than sequence provide the framework for substrate recognition. Comparing multiple states of Microprocessor and the closely related Dicer homologs shed light on the dynamic protein-RNA complex assembly and disassembly required to recognize RNAs with diverse sequences via common structural features.
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U2 - 10.1016/j.sbi.2022.102442
DO - 10.1016/j.sbi.2022.102442
M3 - Review article
C2 - 36067707
AN - SCOPUS:85137061164
SN - 0959-440X
VL - 76
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
M1 - 102442
ER -