Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation

Kvar C.L. Black; Mingzhou Zhou; Pinaki Sarder; Maryna Kuchuk; Amal Y. Al-Yasiri; Sean P. Gunsten; Kexian Liang; Heather M. Hennkens; Walter J. Akers; Richard Laforest; Steven L. Brody; Cathy S. Cutler; Samuel Achilefu

doi:10.1117/12.2301033

Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation

Kvar C.L. Black, Mingzhou Zhou, Pinaki Sarder, Maryna Kuchuk, Amal Y. Al-Yasiri, Sean P. Gunsten, Kexian Liang, Heather M. Hennkens, Walter J. Akers, Richard Laforest, Steven L. Brody, Cathy S. Cutler, Samuel Achilefu

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Quantitative and noninvasive measurement of protease activities has remained an imaging challenge in deep tissues such as the lungs. Here, we designed a dual-radiolabeled probe for reporting the activities of proteases such as matrix metalloproteinases (MMPs) with multispectral single photon emission computed tomography (SPECT) imaging. A gold nanoparticle (NP) was radiolabeled with ¹²⁵I and ¹¹¹In and functionalized with an MMP9-cleavable peptide to form a multispectral SPECT imaging contrast agent. In another design, incorporation of ¹⁹⁹Au radionuclide into the metal crystal structure of gold NPs provided a superior and stable reference signal in lungs, and ¹¹¹In was linked to the NP surface via a protease-cleavable substrate, which can serve as an enzyme activity reporter. This work reveals strategies to correlate protease activities with diverse pathologies in a tissue-depth independent manner.

Original language	English (US)
Title of host publication	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X
Editors	Samuel Achilefu, Ramesh Raghavachari
Publisher	SPIE
ISBN (Electronic)	9781510615014
DOIs	https://doi.org/10.1117/12.2301033
State	Published - 2018
Externally published	Yes
Event	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X 2018 - San Francisco, United States Duration: Jan 29 2018 → Jan 30 2018

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10508
ISSN (Print)	1605-7422

Conference

Conference	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X 2018
Country/Territory	United States
City	San Francisco
Period	1/29/18 → 1/30/18

Keywords

bioimaging
enzyme
in vivo imaging
MMP
nanoparticles
SPECT

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2301033

Cite this

Black, K. C. L., Zhou, M., Sarder, P., Kuchuk, M., Al-Yasiri, A. Y., Gunsten, S. P., Liang, K., Hennkens, H. M., Akers, W. J., Laforest, R., Brody, S. L., Cutler, C. S., & Achilefu, S. (2018). Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation. In S. Achilefu, & R. Raghavachari (Eds.), Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X Article 1050805 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10508). SPIE. https://doi.org/10.1117/12.2301033

Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation. / Black, Kvar C.L.; Zhou, Mingzhou; Sarder, Pinaki et al.
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X. ed. / Samuel Achilefu; Ramesh Raghavachari. SPIE, 2018. 1050805 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10508).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Black, KCL, Zhou, M, Sarder, P, Kuchuk, M, Al-Yasiri, AY, Gunsten, SP, Liang, K, Hennkens, HM, Akers, WJ, Laforest, R, Brody, SL, Cutler, CS & Achilefu, S 2018, Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation. in S Achilefu & R Raghavachari (eds), Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X., 1050805, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10508, SPIE, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X 2018, San Francisco, United States, 1/29/18. https://doi.org/10.1117/12.2301033

Black KCL, Zhou M, Sarder P, Kuchuk M, Al-Yasiri AY, Gunsten SP et al. Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation. In Achilefu S, Raghavachari R, editors, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X. SPIE. 2018. 1050805. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2301033

Black, Kvar C.L. ; Zhou, Mingzhou ; Sarder, Pinaki et al. / Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation. Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X. editor / Samuel Achilefu ; Ramesh Raghavachari. SPIE, 2018. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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title = "Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation",

abstract = "Quantitative and noninvasive measurement of protease activities has remained an imaging challenge in deep tissues such as the lungs. Here, we designed a dual-radiolabeled probe for reporting the activities of proteases such as matrix metalloproteinases (MMPs) with multispectral single photon emission computed tomography (SPECT) imaging. A gold nanoparticle (NP) was radiolabeled with 125I and 111In and functionalized with an MMP9-cleavable peptide to form a multispectral SPECT imaging contrast agent. In another design, incorporation of 199Au radionuclide into the metal crystal structure of gold NPs provided a superior and stable reference signal in lungs, and 111In was linked to the NP surface via a protease-cleavable substrate, which can serve as an enzyme activity reporter. This work reveals strategies to correlate protease activities with diverse pathologies in a tissue-depth independent manner.",

keywords = "bioimaging, enzyme, in vivo imaging, MMP, nanoparticles, SPECT",

author = "Black, {Kvar C.L.} and Mingzhou Zhou and Pinaki Sarder and Maryna Kuchuk and Al-Yasiri, {Amal Y.} and Gunsten, {Sean P.} and Kexian Liang and Hennkens, {Heather M.} and Akers, {Walter J.} and Richard Laforest and Brody, {Steven L.} and Cutler, {Cathy S.} and Samuel Achilefu",

note = "Funding Information: This work was supported in part by grants from the US National Institutes of Health (NCI - U54 CA199092, R01 CA194552, R01 CA152329, P50 CA094056; NHLBI - HHSN268201000046C; NIBIB - R01 EB008111, R01 EB021048), the Department of Defense Breast Cancer Research Program (W81XWH-16-1-0286), and the Alvin J. Siteman Cancer Research Fund (11-FY16-01). Al-Yasiri was supported by the University of Baghdad. We thank the Missouri University Research Reactor for the 199Au synthesis and generously providing the 199Au gold nanocrystal suspensions. We acknowledge Gail Sudlow and LeMoyne Habimana-Griffin for experimental assistance. Publisher Copyright: {\textcopyright} 2018 SPIE.; Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X 2018 ; Conference date: 29-01-2018 Through 30-01-2018",

year = "2018",

doi = "10.1117/12.2301033",

language = "English (US)",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Samuel Achilefu and Ramesh Raghavachari",

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T1 - Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation

AU - Black, Kvar C.L.

AU - Zhou, Mingzhou

AU - Sarder, Pinaki

AU - Kuchuk, Maryna

AU - Al-Yasiri, Amal Y.

AU - Gunsten, Sean P.

AU - Liang, Kexian

AU - Hennkens, Heather M.

AU - Akers, Walter J.

AU - Laforest, Richard

AU - Brody, Steven L.

AU - Cutler, Cathy S.

AU - Achilefu, Samuel

N1 - Funding Information: This work was supported in part by grants from the US National Institutes of Health (NCI - U54 CA199092, R01 CA194552, R01 CA152329, P50 CA094056; NHLBI - HHSN268201000046C; NIBIB - R01 EB008111, R01 EB021048), the Department of Defense Breast Cancer Research Program (W81XWH-16-1-0286), and the Alvin J. Siteman Cancer Research Fund (11-FY16-01). Al-Yasiri was supported by the University of Baghdad. We thank the Missouri University Research Reactor for the 199Au synthesis and generously providing the 199Au gold nanocrystal suspensions. We acknowledge Gail Sudlow and LeMoyne Habimana-Griffin for experimental assistance. Publisher Copyright: © 2018 SPIE.

PY - 2018

Y1 - 2018

N2 - Quantitative and noninvasive measurement of protease activities has remained an imaging challenge in deep tissues such as the lungs. Here, we designed a dual-radiolabeled probe for reporting the activities of proteases such as matrix metalloproteinases (MMPs) with multispectral single photon emission computed tomography (SPECT) imaging. A gold nanoparticle (NP) was radiolabeled with 125I and 111In and functionalized with an MMP9-cleavable peptide to form a multispectral SPECT imaging contrast agent. In another design, incorporation of 199Au radionuclide into the metal crystal structure of gold NPs provided a superior and stable reference signal in lungs, and 111In was linked to the NP surface via a protease-cleavable substrate, which can serve as an enzyme activity reporter. This work reveals strategies to correlate protease activities with diverse pathologies in a tissue-depth independent manner.

AB - Quantitative and noninvasive measurement of protease activities has remained an imaging challenge in deep tissues such as the lungs. Here, we designed a dual-radiolabeled probe for reporting the activities of proteases such as matrix metalloproteinases (MMPs) with multispectral single photon emission computed tomography (SPECT) imaging. A gold nanoparticle (NP) was radiolabeled with 125I and 111In and functionalized with an MMP9-cleavable peptide to form a multispectral SPECT imaging contrast agent. In another design, incorporation of 199Au radionuclide into the metal crystal structure of gold NPs provided a superior and stable reference signal in lungs, and 111In was linked to the NP surface via a protease-cleavable substrate, which can serve as an enzyme activity reporter. This work reveals strategies to correlate protease activities with diverse pathologies in a tissue-depth independent manner.

KW - bioimaging

KW - enzyme

KW - in vivo imaging

KW - MMP

KW - nanoparticles

KW - SPECT

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DO - 10.1117/12.2301033

M3 - Conference contribution

AN - SCOPUS:85047477585

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X

A2 - Achilefu, Samuel

A2 - Raghavachari, Ramesh

PB - SPIE

T2 - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications X 2018

Y2 - 29 January 2018 through 30 January 2018

ER -

Dual-radiolabeled nanoparticle probes for depth-independent in vivo imaging of enzyme activation

Abstract

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Conference

Keywords

ASJC Scopus subject areas

Access to Document

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