TY - JOUR
T1 - DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs
AU - Wada, Tadashi
AU - Takagi, Toshiyuki
AU - Yamaguchi, Yuki
AU - Ferdous, Anwarul
AU - Imai, Takeshi
AU - Hirose, Susumu
AU - Sugimoto, Seiji
AU - Yano, Keiichi
AU - Hartzog, Grant A.
AU - Winston, Fred
AU - Buratowski, Stephen
AU - Handa, Hiroshi
PY - 1998/2/1
Y1 - 1998/2/1
N2 - We report the identification of a transcription elongation factor from HeLa cell nuclear extracts that causes pausing of RNA polymerase II (Pol II) in conjunction with the transcription inhibitor 5,6-dichloro-1-β-D- ribofuranosylbenzimidazole (DRB). This factor, termed DRB sensitivity- inducing factor (DSIF), is also required for transcription inhibition by H8. DSIF has been purified and is composed of 160-kD (p160) and 14-kD (p14) subunits. Isolation of a cDNA encoding DSIF p160 shows it to be a homolog of the Saccharomyces cerevisiae transcription factor Spt5. Recombinant Supt4h protein, the human homolog of yeast Spt4, is functionally equivalent to DSIF p14, indicating that DSIF is composed of the human homologs of Spt4 and Spt5. In addition to its negative role in elongation, DSIF is able to stimulate the rate of elongation by RNA Pol II in a reaction containing limiting concentrations of ribonucleoside triphosphates. A role for DSIF in transcription elongation is further supported by the fact that p160 has a region homologous to the bacterial elongation factor NusG. The combination of biochemical studies on DSIF and genetic analysis of Spt4 and Spt5 in yeast, also in this issue, indicates that DSIF associates with RNA Pol II and regulates its processivity in vitro and in vivo.
AB - We report the identification of a transcription elongation factor from HeLa cell nuclear extracts that causes pausing of RNA polymerase II (Pol II) in conjunction with the transcription inhibitor 5,6-dichloro-1-β-D- ribofuranosylbenzimidazole (DRB). This factor, termed DRB sensitivity- inducing factor (DSIF), is also required for transcription inhibition by H8. DSIF has been purified and is composed of 160-kD (p160) and 14-kD (p14) subunits. Isolation of a cDNA encoding DSIF p160 shows it to be a homolog of the Saccharomyces cerevisiae transcription factor Spt5. Recombinant Supt4h protein, the human homolog of yeast Spt4, is functionally equivalent to DSIF p14, indicating that DSIF is composed of the human homologs of Spt4 and Spt5. In addition to its negative role in elongation, DSIF is able to stimulate the rate of elongation by RNA Pol II in a reaction containing limiting concentrations of ribonucleoside triphosphates. A role for DSIF in transcription elongation is further supported by the fact that p160 has a region homologous to the bacterial elongation factor NusG. The combination of biochemical studies on DSIF and genetic analysis of Spt4 and Spt5 in yeast, also in this issue, indicates that DSIF associates with RNA Pol II and regulates its processivity in vitro and in vivo.
KW - DRB
KW - DSIF
KW - NusG
KW - Protein phosphorylation
KW - Transcription elongation
KW - Transcription elongation factor
KW - Transcription inhibitor
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U2 - 10.1101/gad.12.3.343
DO - 10.1101/gad.12.3.343
M3 - Article
C2 - 9450929
AN - SCOPUS:14444275279
SN - 0890-9369
VL - 12
SP - 343
EP - 356
JO - Genes and Development
JF - Genes and Development
IS - 3
ER -