TY - JOUR
T1 - Dominant expression of a distinctive V14+ T-cell antigen receptor α chain in mice
AU - Koseki, Haruhiko
AU - Asano, Hidefumi
AU - Inaba, Tsuneyoshi
AU - Miyashita, Nobumoto
AU - Moriwaki, Kazuo
AU - Lindahl, Kirsten Fischer
AU - Mizutani, Yoko
AU - Imai, Kenji
AU - Taniguchi, Masaru
PY - 1991
Y1 - 1991
N2 - A distinctive variable region 14-positive (V14+) α chain (Vα14+) of the T-cell antigen receptor is predominantly expressed in multiple mouse subspecies. The Vα14 family has two members, Vα4.1 and Vα14.2, which differ by only three amino acids at positions 50-52. Based on the EcoRI restriction fragment length polymorphism of the gene encoding Vα14, mice can be divided into three groups: type I with an 11.2-kilobase (kb) fragment, type II with a 2.0-kb fragment, and type III with the 2.0-kb and 11.2-kb fragments. Usage of Vα14-Jα281, where Jα281 is an α-chain joining segment, with a one-base N region dominates at the level of 0.02-1.5% of α chains in all laboratory strains, Mus musculus castaneus, and Mus musculus domesticus but not in Mus musculus molossinus, Mus musculus musculus, and Mus spicilegus samples. The preferential Vα14-Jα281 expression seems to be due to positive selection because the V-J junctional region is always glycine, despite the ability of the Vα14 gene to associate with Ja other than Jα281. As Vα14-Jα281 expression is independent of known major histocompatibility complex antigens, including H-2, TLA, Qa, and HMT, the selecting ligand must be a monomorphic molecule of the mouse, expressed in a subspecies-specific manner. Additional observations, such as the expression of homogeneous Vα14-Jα281 in athymic mice, suggest that the positive selection of Vα14+ T cells occurs extrathymically.
AB - A distinctive variable region 14-positive (V14+) α chain (Vα14+) of the T-cell antigen receptor is predominantly expressed in multiple mouse subspecies. The Vα14 family has two members, Vα4.1 and Vα14.2, which differ by only three amino acids at positions 50-52. Based on the EcoRI restriction fragment length polymorphism of the gene encoding Vα14, mice can be divided into three groups: type I with an 11.2-kilobase (kb) fragment, type II with a 2.0-kb fragment, and type III with the 2.0-kb and 11.2-kb fragments. Usage of Vα14-Jα281, where Jα281 is an α-chain joining segment, with a one-base N region dominates at the level of 0.02-1.5% of α chains in all laboratory strains, Mus musculus castaneus, and Mus musculus domesticus but not in Mus musculus molossinus, Mus musculus musculus, and Mus spicilegus samples. The preferential Vα14-Jα281 expression seems to be due to positive selection because the V-J junctional region is always glycine, despite the ability of the Vα14 gene to associate with Ja other than Jα281. As Vα14-Jα281 expression is independent of known major histocompatibility complex antigens, including H-2, TLA, Qa, and HMT, the selecting ligand must be a monomorphic molecule of the mouse, expressed in a subspecies-specific manner. Additional observations, such as the expression of homogeneous Vα14-Jα281 in athymic mice, suggest that the positive selection of Vα14+ T cells occurs extrathymically.
KW - Extrathymic-positive selection
KW - Homogeneous variable-joining region junction
KW - Variable region 14 genes
KW - Wild mice
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M3 - Article
C2 - 1881891
AN - SCOPUS:0025773831
SN - 0027-8424
VL - 88
SP - 7518
EP - 7522
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17
ER -