TY - JOUR
T1 - DNA Polymerases for Translesion DNA Synthesis
T2 - Enzyme Purification and Mouse Models for Studying Their Function
AU - Fischhaber, Paula L.
AU - McDaniel, Lisa D.
AU - Friedberg, Errol C.
PY - 2006
Y1 - 2006
N2 - This chapter discusses experimental methods and protocols for the purification and preliminary characterization of DNA polymerases that are specialized for the replicative bypass (translesion DNA synthesis) of base or other types of DNA damage that typically arrest high-fidelity DNA synthesis, with particular emphasis on DNA polymerase κ (Polκ from mouse cells). It also describes some of the methods employed in the evaluation of mouse strains defective in genes that encode these enzymes.
AB - This chapter discusses experimental methods and protocols for the purification and preliminary characterization of DNA polymerases that are specialized for the replicative bypass (translesion DNA synthesis) of base or other types of DNA damage that typically arrest high-fidelity DNA synthesis, with particular emphasis on DNA polymerase κ (Polκ from mouse cells). It also describes some of the methods employed in the evaluation of mouse strains defective in genes that encode these enzymes.
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U2 - 10.1016/S0076-6879(06)08022-0
DO - 10.1016/S0076-6879(06)08022-0
M3 - Review article
C2 - 16793380
AN - SCOPUS:33746928310
SN - 0076-6879
VL - 408
SP - 355
EP - 378
JO - Methods in Enzymology
JF - Methods in Enzymology
ER -