DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast

Michael A. White; Monika Wierdl; Peter Detloff; Thomas D. Petes

doi:10.1073/pnas.88.21.9755

DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast

Michael A. White, Monika Wierdl, Peter Detloff, Thomas D. Petes

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

In the yeast Saccharomyces cerevisiae, as in other eukaryotes, some regions of the genome have a much higher rate of meiotic recombination than others. We show below that the binding of the RAP1 protein to a site upstream of the HIS4 gene is necessary for a high rate of meiotic (but not mitotic) recombination at this locus. A mutation in the RAP1 binding site at HIS4 results in a decrease in recombination; overproduction of RAP1 causes an increase in recombination at HIS4 above wild-type levels.

Original language	English (US)
Pages (from-to)	9755-9759
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	88
Issue number	21
DOIs	https://doi.org/10.1073/pnas.88.21.9755
State	Published - 1991

Keywords

Saccharomyces cerevisiae
gene conversion
recombination hotspot

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abstract = "In the yeast Saccharomyces cerevisiae, as in other eukaryotes, some regions of the genome have a much higher rate of meiotic recombination than others. We show below that the binding of the RAP1 protein to a site upstream of the HIS4 gene is necessary for a high rate of meiotic (but not mitotic) recombination at this locus. A mutation in the RAP1 binding site at HIS4 results in a decrease in recombination; overproduction of RAP1 causes an increase in recombination at HIS4 above wild-type levels.",

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T1 - DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast

AU - White, Michael A.

AU - Wierdl, Monika

AU - Detloff, Peter

AU - Petes, Thomas D.

PY - 1991

Y1 - 1991

N2 - In the yeast Saccharomyces cerevisiae, as in other eukaryotes, some regions of the genome have a much higher rate of meiotic recombination than others. We show below that the binding of the RAP1 protein to a site upstream of the HIS4 gene is necessary for a high rate of meiotic (but not mitotic) recombination at this locus. A mutation in the RAP1 binding site at HIS4 results in a decrease in recombination; overproduction of RAP1 causes an increase in recombination at HIS4 above wild-type levels.

AB - In the yeast Saccharomyces cerevisiae, as in other eukaryotes, some regions of the genome have a much higher rate of meiotic recombination than others. We show below that the binding of the RAP1 protein to a site upstream of the HIS4 gene is necessary for a high rate of meiotic (but not mitotic) recombination at this locus. A mutation in the RAP1 binding site at HIS4 results in a decrease in recombination; overproduction of RAP1 causes an increase in recombination at HIS4 above wild-type levels.

KW - Saccharomyces cerevisiae

KW - gene conversion

KW - recombination hotspot

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JF - Proceedings of the National Academy of Sciences of the United States of America

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DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast

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Keywords

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