TY - JOUR
T1 - Distinct neuronal circuits mediate experience-dependent, non-associative osmotactic responses in Drosophila
AU - Acevedo, Summer F.
AU - Froudarakis, Emmanuil I.
AU - Tsiorva, Anna Angeliki
AU - Skoulakis, Efthimios M C
N1 - Funding Information:
We would like to thank Drs. Gregg Roman, Sumana Datta and all members of the lab for their valuable discussions and suggestions, Maria Anezaki, Courtney Swayze, and Alexandros Kanellopoulos for stock maintenance and husbandry. This work was supported by National Science Foundation Grant IBN-0080687 and the European Commission Marie Curie IRG-CT-2004-003570.
PY - 2007/3
Y1 - 2007/3
N2 - Osmotactic responses can be modified in an experience-dependent manner and have been used to condition animals in negative or positive associative paradigms. Experience-dependent non-associative defects in avoidance of aversive odors were reported in Drosophila learning mutants. This prompted an examination of the contribution of the mushroom bodies and inner antenoglomerular tract, the two neuronal populations implicated in processing olfactory information to experience-dependent non-associative osmotactic responses. Silencing inner antenoglomerular tract synapses results in defective osmotaxis after experiencing a different odor, but not electric shock. Conversely, structural or functional perturbation of the mushroom bodies precipitates osmotactic deficits after prior experience of electric shock but not odors. These effects on osmotaxis are specific, long lasting and independent of the aversive or attractive properties of the odors. Deficient osmotactic responses only after electric shock stimulation were exhibited by mutants with altered cAMP levels, but all other mutants in genes preferentially expressed in the mushroom bodies responded normally. Our results suggest that the mushroom bodies and inner antenoglomerular tract are essential for normal osmotactic responses after prior stimulation with electric shock or another odor respectively. Finally, these experience-dependent non-associative paradigms are excellent methods of functionally ascertaining normal activity of the mushroom bodies and inner antenoglomerular tract in putative leaning and memory mutants.
AB - Osmotactic responses can be modified in an experience-dependent manner and have been used to condition animals in negative or positive associative paradigms. Experience-dependent non-associative defects in avoidance of aversive odors were reported in Drosophila learning mutants. This prompted an examination of the contribution of the mushroom bodies and inner antenoglomerular tract, the two neuronal populations implicated in processing olfactory information to experience-dependent non-associative osmotactic responses. Silencing inner antenoglomerular tract synapses results in defective osmotaxis after experiencing a different odor, but not electric shock. Conversely, structural or functional perturbation of the mushroom bodies precipitates osmotactic deficits after prior experience of electric shock but not odors. These effects on osmotaxis are specific, long lasting and independent of the aversive or attractive properties of the odors. Deficient osmotactic responses only after electric shock stimulation were exhibited by mutants with altered cAMP levels, but all other mutants in genes preferentially expressed in the mushroom bodies responded normally. Our results suggest that the mushroom bodies and inner antenoglomerular tract are essential for normal osmotactic responses after prior stimulation with electric shock or another odor respectively. Finally, these experience-dependent non-associative paradigms are excellent methods of functionally ascertaining normal activity of the mushroom bodies and inner antenoglomerular tract in putative leaning and memory mutants.
KW - Drosophila
KW - Experience-dependent behaviors
KW - Olfactory conditioning
KW - Osmotaxis
KW - cAMP
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U2 - 10.1016/j.mcn.2006.11.011
DO - 10.1016/j.mcn.2006.11.011
M3 - Article
C2 - 17197197
AN - SCOPUS:33847387495
SN - 1044-7431
VL - 34
SP - 378
EP - 389
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 3
ER -