@inbook{7288212a7d834bdeaa14970750a5d0b8,
title = "Dissecting Locus-Specific Chromatin Interactions by CRISPR CAPTURE",
abstract = "The spatiotemporal control of tissue-specific gene expression is coordinated by cis-regulatory elements (CREs) and associated trans-acting factors. Despite major advances in genome-wide annotation of candidate CREs, the in situ regulatory composition of the vast majority of CREs remain unknown. To address this challenge, we developed the CRISPR affinity purification in situ of regulatory elements (CAPTURE) toolbox that employs an in vivo biotinylated nuclease-deficient Cas9 (dCas9) protein and programmable single-guide RNAs (sgRNAs) to identify CRE-associated macromolecular complexes and chromatin looping. In this chapter, we provide a detailed protocol for implementing the latest iteration of the CRISPR-based CAPTURE methods to interrogate the molecular composition of locus-specific chromatin complexes and configuration in a mammalian genome.",
keywords = "Biotinylated dCas9, CAPTURE, CAPTURE-3C-seq, CAPTURE-Proteomics, Chromatin looping, cis-regulatory elements, CRISPR, Enhancer, Gene regulation, Noncoding genome",
author = "Botten, {Giovanni A.} and Michael Lee and Jian Xu",
note = "Funding Information: We thank Drs. Xin Liu, Yuannyu Zhang, Yong Chen, Mushan Li, Zhen Shao, and Michael Q. Zhang for their help with the assay development and/or data analysis pipelines, Yoon Jung Kim at the Children{\textquoteright}s Research Institute (CRI) Sequencing Core Facility for assistance with next-generation sequencing, and other Xu laboratory members for discussion and technical support. G.A.B. was supported by the American Heart Association (AHA) Predoctoral Fellowship (827234). J.X. is a Scholar of The Leukemia & Lymphoma Society (LLS) and an American Society of Hematology (ASH) Scholar. This work was supported by the NIH grants R01DK111430, R01CA230631, R01CA259581, and R21AI158240 (to J.X.), by the CPRIT grants (RP180504 and RP190417 to J.X.), and by the Welch Foundation grants I-1942 (to J.X.). Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2847-8_7",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "69--97",
booktitle = "Methods in Molecular Biology",
}