TY - JOUR
T1 - Dissecting lipid metabolism in meibomian glands of humans and mice
T2 - An integrative study reveals a network of metabolic reactions not duplicated in other tissues
AU - Butovich, Igor A.
AU - McMahon, Anne
AU - Wojtowicz, Jadwiga C.
AU - Lin, Feng
AU - Mancini, Ronald
AU - Itani, Kamel
N1 - Publisher Copyright:
© 2016 Elsevier B.V. All rights reserved.
PY - 2016/6
Y1 - 2016/6
N2 - Lipids comprise the bulk of the meibomian gland secretion (meibum) which is produced by meibocytes. Complex arrays of lipogenic reactions in meibomian glands, which we collectively call meibogenesis, have not been explored on a molecular level yet. Our goals were to elucidate the possible biosynthetic pathways that underlie the generation of meibum, reveal similarities in, and differences between, lipid metabolism in meibomian glands and other organs and tissues, and integrate meibomian gland studies into the field of general metabolomics. Specifically, we have conducted detailed analyses of human and mouse specimens using genomic, immunohistochemical, and lipidomic approaches. Among equally highly expressed genes found in meibomian glands of both species were those related to fatty acid elongation, branching, desaturation, esterification, reduction of fatty acids to alcohols, and cholesterol biosynthesis. Importantly, corresponding lipid products were detected in meibum of both species using lipidomic approaches. For the first time, a cohesive, unifying biosynthetic scheme that connects genomic, lipidomic, and immunohistochemical observations is outlined and discussed.
AB - Lipids comprise the bulk of the meibomian gland secretion (meibum) which is produced by meibocytes. Complex arrays of lipogenic reactions in meibomian glands, which we collectively call meibogenesis, have not been explored on a molecular level yet. Our goals were to elucidate the possible biosynthetic pathways that underlie the generation of meibum, reveal similarities in, and differences between, lipid metabolism in meibomian glands and other organs and tissues, and integrate meibomian gland studies into the field of general metabolomics. Specifically, we have conducted detailed analyses of human and mouse specimens using genomic, immunohistochemical, and lipidomic approaches. Among equally highly expressed genes found in meibomian glands of both species were those related to fatty acid elongation, branching, desaturation, esterification, reduction of fatty acids to alcohols, and cholesterol biosynthesis. Importantly, corresponding lipid products were detected in meibum of both species using lipidomic approaches. For the first time, a cohesive, unifying biosynthetic scheme that connects genomic, lipidomic, and immunohistochemical observations is outlined and discussed.
KW - Branched lipids
KW - Extremely long chain lipids
KW - Gene expression
KW - Lipidomics
KW - Meibocytes
KW - Meibogenesis
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UR - http://www.scopus.com/inward/citedby.url?scp=84963574893&partnerID=8YFLogxK
U2 - 10.1016/j.bbalip.2016.03.024
DO - 10.1016/j.bbalip.2016.03.024
M3 - Article
C2 - 27032494
AN - SCOPUS:84963574893
SN - 1388-1981
VL - 1861
SP - 538
EP - 553
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 6
ER -