Disruption of the yeast N-myristoyl transferase gene causes recessive lethality

Robert J. Duronio; Dwight A. Towler; Robert O. Heuckeroth; Jeffrey I. Gordon

doi:10.1126/science.2644694

Disruption of the yeast N-myristoyl transferase gene causes recessive lethality

Robert J. Duronio, Dwight A. Towler, Robert O. Heuckeroth, Jeffrey I. Gordon

Research output: Contribution to journal › Article › peer-review

186 Scopus citations

Abstract

The structural gene for N-myristoyl transferase (NMT1) has been cloned from the budding yeast Saccharomyces cerevisiae. The gene encodes a polypeptide of 455 amino acids (M_r = 52,837) that has no identifiable significant primary sequence homology with any protein in currently available databases. Overexpression of NMT activity was achieved by means of the yeast episomal plasmid YEp24 without obvious effects on growth kinetics, cell morphology, or acylprotein metabolic labeling patterns. Insertional mutagenesis of the NMT1 locus on yeast chromosome XII caused recessive lethality, indicating that this protein acyltransferase activity is necessary for vegetative cell growth.

Original language	English (US)
Pages (from-to)	796-800
Number of pages	5
Journal	Science
Volume	243
Issue number	4892
DOIs	https://doi.org/10.1126/science.2644694
State	Published - 1989

ASJC Scopus subject areas

General

Access to Document

10.1126/science.2644694

Cite this

@article{709bc8484b0e4a0ca2e5fe65e2636b19,

title = "Disruption of the yeast N-myristoyl transferase gene causes recessive lethality",

abstract = "The structural gene for N-myristoyl transferase (NMT1) has been cloned from the budding yeast Saccharomyces cerevisiae. The gene encodes a polypeptide of 455 amino acids (Mr = 52,837) that has no identifiable significant primary sequence homology with any protein in currently available databases. Overexpression of NMT activity was achieved by means of the yeast episomal plasmid YEp24 without obvious effects on growth kinetics, cell morphology, or acylprotein metabolic labeling patterns. Insertional mutagenesis of the NMT1 locus on yeast chromosome XII caused recessive lethality, indicating that this protein acyltransferase activity is necessary for vegetative cell growth.",

author = "Duronio, {Robert J.} and Towler, {Dwight A.} and Heuckeroth, {Robert O.} and Gordon, {Jeffrey I.}",

year = "1989",

doi = "10.1126/science.2644694",

language = "English (US)",

volume = "243",

pages = "796--800",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4892",

}

TY - JOUR

T1 - Disruption of the yeast N-myristoyl transferase gene causes recessive lethality

AU - Duronio, Robert J.

AU - Towler, Dwight A.

AU - Heuckeroth, Robert O.

AU - Gordon, Jeffrey I.

PY - 1989

Y1 - 1989

N2 - The structural gene for N-myristoyl transferase (NMT1) has been cloned from the budding yeast Saccharomyces cerevisiae. The gene encodes a polypeptide of 455 amino acids (Mr = 52,837) that has no identifiable significant primary sequence homology with any protein in currently available databases. Overexpression of NMT activity was achieved by means of the yeast episomal plasmid YEp24 without obvious effects on growth kinetics, cell morphology, or acylprotein metabolic labeling patterns. Insertional mutagenesis of the NMT1 locus on yeast chromosome XII caused recessive lethality, indicating that this protein acyltransferase activity is necessary for vegetative cell growth.

AB - The structural gene for N-myristoyl transferase (NMT1) has been cloned from the budding yeast Saccharomyces cerevisiae. The gene encodes a polypeptide of 455 amino acids (Mr = 52,837) that has no identifiable significant primary sequence homology with any protein in currently available databases. Overexpression of NMT activity was achieved by means of the yeast episomal plasmid YEp24 without obvious effects on growth kinetics, cell morphology, or acylprotein metabolic labeling patterns. Insertional mutagenesis of the NMT1 locus on yeast chromosome XII caused recessive lethality, indicating that this protein acyltransferase activity is necessary for vegetative cell growth.

UR - http://www.scopus.com/inward/record.url?scp=0024590280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024590280&partnerID=8YFLogxK

U2 - 10.1126/science.2644694

DO - 10.1126/science.2644694

M3 - Article

C2 - 2644694

AN - SCOPUS:0024590280

SN - 0036-8075

VL - 243

SP - 796

EP - 800

JO - Science

JF - Science

IS - 4892

ER -

Disruption of the yeast N-myristoyl transferase gene causes recessive lethality

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this