Dihydrostreptomycin Directly Binds to, Modulates, and Passes through the MscL Channel Pore

Robin Wray, Irene Iscla, Ya Gao, Hua Li, Junmei Wang, Paul Blount

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


The primary mechanism of action of the antibiotic dihydrostreptomycin is binding to and modifying the function of the bacterial ribosome, thus leading to decreased and aberrant translation of proteins; however, the routes by which it enters the bacterial cell are largely unknown. The mechanosensitive channel of large conductance, MscL, is found in the vast majority of bacterial species, where it serves as an emergency release valve rescuing the cell from sudden decreases in external osmolarity. While it is known that MscL expression increases the potency of dihydrostreptomycin, it has remained unclear if this effect is due to a direct interaction. Here, we use a combination of genetic screening, MD simulations, and biochemical and mutational approaches to determine if dihydrostreptomycin directly interacts with MscL. Our data strongly suggest that dihydrostreptomycin binds to a specific site on MscL and modifies its conformation, thus allowing the passage of K+ and glutamate out of, and dihydrostreptomycin into, the cell.

Original languageEnglish (US)
Article numbere1002473
JournalPLoS biology
Issue number6
StatePublished - Jun 9 2016

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • General Agricultural and Biological Sciences


Dive into the research topics of 'Dihydrostreptomycin Directly Binds to, Modulates, and Passes through the MscL Channel Pore'. Together they form a unique fingerprint.

Cite this