@article{3c1184a41e9b4ff8afbe4524b7901efd,
title = "Differential cell composition and split epidermal differentiation in human palm, sole, and hip skin",
abstract = "Palmoplantar skin is structurally and functionally unique, but the transcriptional programs driving this specialization are unclear. Here, we use bulk and single-cell RNA sequencing of human palm, sole, and hip skin to describe the distinguishing characteristics of palmoplantar and non-palmoplantar skin while also uncovering differences between palmar and plantar sites. Our approach reveals an altered immune environment in palmoplantar skin, with downregulation of diverse immunological processes and decreased immune cell populations. Further, we identify specific fibroblast populations that appear to orchestrate key differences in cell-cell communication in palm, sole, and hip. Dedicated keratinocyte analysis highlights major differences in basal cell fraction among the three sites and demonstrates the existence of two spinous keratinocyte populations constituting parallel, site-selective epidermal differentiation trajectories. In summary, this deep characterization of highly adapted palmoplantar skin contributes key insights into the fundamental biology of human skin and provides a valuable data resource for further investigation.",
keywords = "CP: Cell biology, epidermal differentation, fibroblast, human epidermis, keratinocyte, palm, palmoplantar skin, RNA FISH, single-cell RNA sequencing, skin, sole",
author = "Julie Wiedemann and Billi, {Allison C.} and Federico Bocci and Ghaidaa Kashgari and Enze Xing and Tsoi, {Lam C.} and Leo Meller and Swindell, {William R.} and Rachael Wasikowski and Xianying Xing and Feiyang Ma and Mehrnaz Gharaee-Kermani and Kahlenberg, {J. Michelle} and Harms, {Paul W.} and Emanual Maverakis and Qing Nie and Gudjonsson, {Johann E.} and Bogi Andersen",
note = "Funding Information: J.M.K. has received grant support from Q32 Bio, Celgene/BMS, Ventus Therapeutics, and Janssen. J.M.K. has served on advisory boards for AstraZeneca, Eli Lilly, GlaxoSmithKline, Bristol Myers Squibb, Avion Pharmaceuticals, ProventionBio, Aurinia Pharmaceuticals, Ventus Therapeutics, and Boehringer Ingelheim. J.E.G. has received grant support from Celgene/BMS, Janssen, Eli Lilly, and Almirall. J.E.G. has served on advisory boards for AstraZeneca, Sanofi, Eli Lilly, Boehringer Ingelheim, Novartis, Janssen, Almirall, and BMS. Funding Information: This work was supported by NIH grants P30AR075047 and R01AR44882 and the Irving Weinstein Foundation (to B.A.); an NSF grant DMS1763272 and a grant from the Simons Foundation ( 594598 ) (Q.N.); and NIH grants K08AR078251 (A.C.B.), R01AR069071 (J.E.G.), R01AR073196 (J.E.G.), P30AR075043 (J.E.G.), R01AR071384 (J.M.K.), K24AR076975 (J.M.K.), and K01AR072129 (L.C.T.). This work was additionally supported by Chan Zuckerberg Initiative grant DAF2022-239946 (B.A., A.C.B, J.G., J.M.K., Q.N., and L.C.T.); Taubman Institute via Innovative Program (J.E.G. and J.M.K.); Parfet Emerging Scholar (J.M.K.) and Wexner Emerging Scholar (A.C.B.) funds; the Lupus Research Alliance (J.M.K.); the Dermatology Foundation (J.E.G. via the Sun Pharma Research Award, A.C.B., and L.C.T.); the Arthritis National Research Foundation (L.C.T.); and the National Psoriasis Foundation (L.C.T.). P.W.H. receives support from CA046592 ( University of Michigan Rogel Cancer Center Support Grant) and AR075043 (University of Michigan Skin Biology and Diseases Resource-Based Center); E.X. receives support from the Blue Cross Blue Shield Foundation of Michigan (Student Award) and the University of Michigan Rackham Graduate School. J.W. was supported by T32-GM136624 from the National Institute of General Medical Sciences . Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
month = jan,
day = "31",
doi = "10.1016/j.celrep.2023.111994",
language = "English (US)",
volume = "42",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "1",
}