Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila

Feng Jiang, Xuecheng Ye, Xiang Liu, Lauren Fincher, Dennis McKearin, Qinghua Liu

Research output: Contribution to journalArticlepeer-review

240 Scopus citations


In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNAbinding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.

Original languageEnglish (US)
Pages (from-to)1674-1679
Number of pages6
JournalGenes and Development
Issue number14
StatePublished - Jul 2005


  • Dicer-1
  • Pre-miRNA
  • R3D1-L
  • RNase III
  • dsRNA binding
  • miRNA

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology


Dive into the research topics of 'Dicer-1 and R3D1-L catalyze microRNA maturation in Drosophila'. Together they form a unique fingerprint.

Cite this