Dibutyryl cAMP-induced protein changes in differentiating mouse neuroblastoma cells

N. Prashad; B. Wischmeyer; C. Evetts; F. Baskin; R. Rosenberg

doi:10.1016/0045-6039(77)90036-7

Dibutyryl cAMP-induced protein changes in differentiating mouse neuroblastoma cells

N. Prashad, B. Wischmeyer, C. Evetts, F. Baskin, R. Rosenberg

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Proteins from mouse neuroblastoma cells treated with dibutyryl adenosine-3′,5′-monophosphate (B₂cAMP) were analyzed by high resolution, two-dimensional gel electrophoresic. Quantitative changes in proteins and charge modifications of proteins apparently induced B₂cAMP were detected by isoelectric focusing. Some proteins appeared to be modified and one protein was increased 7- to 8-fold in cells treated with B₂cAMP. Since neuroblastoma cells differentiate when treated with B₂cAMP, understanding the protein changes induced by B₂cAMP may help to understand cellular differentiation in neural tissue.

Original language	English (US)
Pages (from-to)	147-157
Number of pages	11
Journal	Cell Differentiation
Volume	6
Issue number	2
DOIs	https://doi.org/10.1016/0045-6039(77)90036-7
State	Published - Aug 1977

ASJC Scopus subject areas

Developmental Biology

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10.1016/0045-6039(77)90036-7

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title = "Dibutyryl cAMP-induced protein changes in differentiating mouse neuroblastoma cells",

abstract = "Proteins from mouse neuroblastoma cells treated with dibutyryl adenosine-3′,5′-monophosphate (B2cAMP) were analyzed by high resolution, two-dimensional gel electrophoresic. Quantitative changes in proteins and charge modifications of proteins apparently induced B2cAMP were detected by isoelectric focusing. Some proteins appeared to be modified and one protein was increased 7- to 8-fold in cells treated with B2cAMP. Since neuroblastoma cells differentiate when treated with B2cAMP, understanding the protein changes induced by B2cAMP may help to understand cellular differentiation in neural tissue.",

author = "N. Prashad and B. Wischmeyer and C. Evetts and F. Baskin and R. Rosenberg",

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T1 - Dibutyryl cAMP-induced protein changes in differentiating mouse neuroblastoma cells

AU - Prashad, N.

AU - Wischmeyer, B.

AU - Evetts, C.

AU - Baskin, F.

AU - Rosenberg, R.

N1 - Funding Information: W.-* th~tk t.~rsH. . Feig and A.J. ~d,~arz-Praahfaodr ['~C]tubulhã~ d ['~I]actin aampleS ~nd.I -~:.M. Morri~onA, .J. ~ed~rz-Pr~imadnd H. FeLt for i,elpful corn,--ne~,.ts. Supported by Leland ~]haa FoundationIn, c. a~dG ra~tN I}! CA 17665--02.

PY - 1977/8

Y1 - 1977/8

N2 - Proteins from mouse neuroblastoma cells treated with dibutyryl adenosine-3′,5′-monophosphate (B2cAMP) were analyzed by high resolution, two-dimensional gel electrophoresic. Quantitative changes in proteins and charge modifications of proteins apparently induced B2cAMP were detected by isoelectric focusing. Some proteins appeared to be modified and one protein was increased 7- to 8-fold in cells treated with B2cAMP. Since neuroblastoma cells differentiate when treated with B2cAMP, understanding the protein changes induced by B2cAMP may help to understand cellular differentiation in neural tissue.

AB - Proteins from mouse neuroblastoma cells treated with dibutyryl adenosine-3′,5′-monophosphate (B2cAMP) were analyzed by high resolution, two-dimensional gel electrophoresic. Quantitative changes in proteins and charge modifications of proteins apparently induced B2cAMP were detected by isoelectric focusing. Some proteins appeared to be modified and one protein was increased 7- to 8-fold in cells treated with B2cAMP. Since neuroblastoma cells differentiate when treated with B2cAMP, understanding the protein changes induced by B2cAMP may help to understand cellular differentiation in neural tissue.

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JF - Cell Differentiation

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ER -

Dibutyryl cAMP-induced protein changes in differentiating mouse neuroblastoma cells

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