Development of a polarized hyperspectral microscope for cardiac fiber orientation imaging

Ximing Zhou, James Dormer, Baowei Fei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Myocardial fiber orientation is closely related to the functions of the heart. The development of imaging tools for depicting myocardial fiber orientation is important. We developed a polarized hyperspectral imaging microscope (PHSIM) for cardiac fiber orientation imaging, which is capable of polarimetric imaging and hyperspectral imaging. Polarimetric imaging is realized by the integration of two polarizers. Hyperspectral imaging is realized by snapscan Preliminary imaging experiments were implemented on an unstained paraffin embedded tißue slides of a chicken heart. We also set up a Monte Carlo simulation program based on the cylinder optical model to simulate the cardiac fiber structure of the sample and the optical setup of the PHSIM system, in which we can calculate the system output light intensity related to cardiac fiber orientation. According to the imaging and simulation results, there exists a variation of intensity of acquired images with the polar angles from the maximum to the minimum under different wavelengths, which should relate to the orientation of cardiac fibers. In addition, there is a shift of the polar angle where the maximum intensity appears when a rotation of the sample happened both in the simulation and imaging experiments. Further work is required for imaging more types of myocardial tißues at different parts and the design of a complete quantitative model to describe the relations among polar angles, wavelengths, and cardiac fiber orientations.

Original languageEnglish (US)
Title of host publicationDiagnostic and Therapeutic Applications of Light in Cardiology 2020
EditorsKenton W. Gregory, Laura Marcu
ISBN (Electronic)9781510631939
StatePublished - 2020
EventDiagnostic and Therapeutic Applications of Light in Cardiology 2020 - San Francisco, United States
Duration: Feb 1 2020Feb 2 2020

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceDiagnostic and Therapeutic Applications of Light in Cardiology 2020
Country/TerritoryUnited States
CitySan Francisco


  • Cardiac fiber
  • Hyperspectral imaging
  • Monte Carlo simulation
  • Polarized light imaging
  • Stokes vector

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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