Determinants of clathrin-coated vesicle acidification.

X. S. Xie, D. K. Stone, E. Racker

Research output: Contribution to journalArticlepeer-review

71 Scopus citations


The proton-translocating ATPase of clathrin-coated vesicles of bovine brain is characterized by ATP specificity. Chloride or bromide serve as co-ions balancing electrogenic proton pumping. ATP-driven proton translocation can be observed in the absence of chloride, provided the membrane potential is collapsed by K+ moving out in the presence of valinomycin. Chloride transport can be observed independent of proton movements in the absence of ATP, provided an inside positive membrane potential is generated by K+ moving from the outside to the inside in the presence of valinomycin.

Original languageEnglish (US)
Pages (from-to)14834-14838
Number of pages5
JournalJournal of Biological Chemistry
Issue number24
StatePublished - Dec 25 1983

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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