Design and Characterization of Hyperpolarized 15N-BBCP as a H2O2-Sensing Probe

Hyejin Park, Jun Chen, Ivan E. Dimitrov, Jae Mo Park, Qiu Wang

Research output: Contribution to journalArticlepeer-review


Hydrogen peroxide (H2O2) is a type of reactive oxygen species that regulates essential biological processes. Despite the central role of H2O2 in pathophysiological states, available molecular probes for assessing H2O2 in vivo are still limited. This work develops hyperpolarized 15N-boronobenzyl-4-cyanopyridinium (15N-BBCP) as a rationally designed molecular probe for detecting H2O2. The 15N-BBCP demonstrated favorable physicochemical and biochemical properties for H2O2 detection and dynamic nuclear polarization, allowing noninvasive detection of H2O2. In particular, 15N-BBCP and the products possessed long spin-lattice relaxation times and spectrally resolvable 15N chemical shift differences. The performance of hyperpolarized 15N-BBCP was demonstrated both in vitro and in vivo with time-resolved 15N-MRS. This study highlights a promising approach to designing a reaction-based 15N-labeled molecular imaging agent for detecting oxidative stress in vivo.

Original languageEnglish (US)
Pages (from-to)2928-2933
Number of pages6
JournalACS Sensors
Issue number10
StatePublished - Oct 28 2022
Externally publishedYes


  • DNP hyperpolarization
  • HO-sensing
  • magnetic resonance spectroscopy and imaging
  • nitrogen-15-NMR
  • reaction-based probe

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes


Dive into the research topics of 'Design and Characterization of Hyperpolarized 15N-BBCP as a H2O2-Sensing Probe'. Together they form a unique fingerprint.

Cite this