Dependence of blood T2 on oxygenation at 7 T: In vitro calibration and in vivo application

Lisa C. Krishnamurthy, Peiying Liu, Feng Xu, Jinsoo Uh, Ivan Dimitrov, Hanzhang Lu

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Purpose: The calibratable relationship between blood oxygenation (Y) and T2 allows quantification of cerebral venous oxygenation. We aim to establish a calibration plot between blood T2, Y, and hematocrit at 7 T, and using T2-relaxation-under-spin-tagging MRI, determine human venous blood oxygenation in vivo. Methods: In vitro experiments were performed at 7 T on bovine blood samples using a Carr-Purcell-Meiboom-Gill-T2 sequence, from which we characterized the relationship among T2, Y, and hematocrit. T2-relaxation-under-spin-tagging MRI was implemented at 7 T to measure venous blood T2 in vivo, from which oxygenation was estimated using the in vitro calibration plot. Hyperoxia was performed to test the sensitivity of the method to oxygenation changes, and the 7 T results were compared with those at 3 T. Results: In vitro data showed that arterial and venous T2 at 7 T are 68 and 20 ms, respectively, at a typical hematocrit of 0.42. In vivo measurement showed a cerebral venous oxygenation of 64.7 ± 5.0% and a test-retest coefficient-of-variation of 3.6 ± 2.4%. Hyperoxia increased Yv by 9.0 ± 1.4% (P = 0.001) and the 3 and 7 T results showed a strong correlation (R = 0.95) across individuals. Conclusion: We provided an in vitro calibration plot for conversion of blood T2 to oxygenation at 7 T and demonstrated its utility in vivo.

Original languageEnglish (US)
Pages (from-to)2035-2042
Number of pages8
JournalMagnetic resonance in medicine
Issue number6
StatePublished - Jun 2014


  • blood oxygen saturation
  • brain

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


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