Dating bloodstains with fluorescence lifetime measurements

Kevin Guo, Samuel Achilefu, Mikhail Y. Berezin

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


In summary, fluorescence lifetime was shown to be a promising technique to evaluate the age of a bloodstain in a crime scene within the first week. The method is fast (ca. 20 min), reproducible, concentration independent, and requires a miniscule amount of the bloodstain with minimal sample preparation. The major improvement of the technique will come from the development of the lifetime imaging technique, in which the average fluorescence lifetime is evaluated across the region of interest in the bloodstain. Such an approach will minimize the problems with sampling and provide more accurate results. The measurements can potentially be performed in solid state by using a handheld fluorescence-lifetime instrument, which could be applied to the bloodstain directly at the crime scene with no additional sample handling.

Original languageEnglish (US)
Pages (from-to)1303-1305
Number of pages3
JournalChemistry - A European Journal
Issue number5
StatePublished - Jan 27 2012
Externally publishedYes


  • Bloodstain age
  • Fluorescence lifetime
  • Forensics
  • Tryptophan

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry


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