Damage-induced regeneration of the intestinal stem cell pool through enteroblast mitosis in the Drosophila midgut

Aiguo Tian, Virginia Morejon, Sarah Kohoutek, Yi Chun Huang, Wu Min Deng, Jin Jiang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Many adult tissues and organs including the intestine rely on resident stem cells to maintain homeostasis and regeneration. In mammals, the progenies of intestinal stem cells (ISCs) can dedifferentiate to generate ISCs upon ablation of resident stem cells. However, whether and how mature tissue cells generate ISCs under physiological conditions remains unknown. Here, we show that infection of the Drosophila melanogaster intestine with pathogenic bacteria induces entry of enteroblasts (EBs), which are ISC progenies, into the mitotic cycle through upregulation of epidermal growth factor receptor (EGFR)-Ras signaling. We also show that ectopic activation of EGFR-Ras signaling in EBs is sufficient to drive enteroblast mitosis cell autonomously. Furthermore, we find that the dividing enteroblasts do not gain ISC identity as a prerequisite to divide, and the regenerative ISCs are produced through EB mitosis. Taken together, our work uncovers a new role for EGFR-Ras signaling in driving EB mitosis and replenishing the ISC pool during fly intestinal regeneration, which may have important implications for tissue homeostasis and tumorigenesis in vertebrates.

Original languageEnglish (US)
Article numbere110834
JournalEMBO Journal
Issue number19
StatePublished - Oct 4 2022


  • Drosophila midgut
  • dedifferentiation
  • enteroblasts
  • regeneration
  • stem cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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