D-Aspartate activates mGlu receptors coupled to polyphosphoinositide hydrolysis in neonate rat brain slices

Gemma Molinaro; Sara Pietracupa; Luisa Di Menna; Lorenzo Pescatori; Alessandro Usiello; Giuseppe Battaglia; Ferdinando Nicoletti; Valeria Bruno

doi:10.1016/j.neulet.2010.04.077

D-Aspartate activates mGlu receptors coupled to polyphosphoinositide hydrolysis in neonate rat brain slices

Gemma Molinaro, Sara Pietracupa, Luisa Di Menna, Lorenzo Pescatori, Alessandro Usiello, Giuseppe Battaglia, Ferdinando Nicoletti, Valeria Bruno

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

The d-amino acid, d-aspartate, is abundant in the developing brain, yet its function is unknown. Addition of d-aspartate to hippocampal or cortical slices prepared from 8- to 9-day-old rats stimulated polyphosphoinositide (PI) hydrolysis to a slightly greater extent than l-glutamate. The action of d-aspartate was concentration-dependent with an apparent EC₅₀ value of 1mM and a maximal stimulation of 6- and 20-fold in cortical and hippocampal slices, respectively. Stimulation of PI hydrolysis by d-aspartate was largely reduced by pharmacological blockade of mGlu5 metabotropic glutamate receptors with 2-methyl-6-(phenylethynyl)pyridine. These findings suggest that d-aspartate behaves as an endogenous agonist of mGlu5 receptors during early postnatal life.

Original language	English (US)
Pages (from-to)	128-130
Number of pages	3
Journal	Neuroscience letters
Volume	478
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2010.04.077
State	Published - Jul 2010

Keywords

Cortical slices
D-Aspartate
Hippocampal slices
Metabotropic glutamate receptors
Polyphosphoinositide hydrolysis

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2010.04.077

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title = "D-Aspartate activates mGlu receptors coupled to polyphosphoinositide hydrolysis in neonate rat brain slices",

abstract = "The d-amino acid, d-aspartate, is abundant in the developing brain, yet its function is unknown. Addition of d-aspartate to hippocampal or cortical slices prepared from 8- to 9-day-old rats stimulated polyphosphoinositide (PI) hydrolysis to a slightly greater extent than l-glutamate. The action of d-aspartate was concentration-dependent with an apparent EC50 value of 1mM and a maximal stimulation of 6- and 20-fold in cortical and hippocampal slices, respectively. Stimulation of PI hydrolysis by d-aspartate was largely reduced by pharmacological blockade of mGlu5 metabotropic glutamate receptors with 2-methyl-6-(phenylethynyl)pyridine. These findings suggest that d-aspartate behaves as an endogenous agonist of mGlu5 receptors during early postnatal life.",

keywords = "Cortical slices, D-Aspartate, Hippocampal slices, Metabotropic glutamate receptors, Polyphosphoinositide hydrolysis",

author = "Gemma Molinaro and Sara Pietracupa and {Di Menna}, Luisa and Lorenzo Pescatori and Alessandro Usiello and Giuseppe Battaglia and Ferdinando Nicoletti and Valeria Bruno",

note = "Funding Information: This work was supported by Fondazione Telethon , Project No. GGP07264A to F.N.",

year = "2010",

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doi = "10.1016/j.neulet.2010.04.077",

language = "English (US)",

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AU - Molinaro, Gemma

AU - Pietracupa, Sara

AU - Di Menna, Luisa

AU - Pescatori, Lorenzo

AU - Usiello, Alessandro

AU - Battaglia, Giuseppe

AU - Nicoletti, Ferdinando

AU - Bruno, Valeria

N1 - Funding Information: This work was supported by Fondazione Telethon , Project No. GGP07264A to F.N.

PY - 2010/7

Y1 - 2010/7

N2 - The d-amino acid, d-aspartate, is abundant in the developing brain, yet its function is unknown. Addition of d-aspartate to hippocampal or cortical slices prepared from 8- to 9-day-old rats stimulated polyphosphoinositide (PI) hydrolysis to a slightly greater extent than l-glutamate. The action of d-aspartate was concentration-dependent with an apparent EC50 value of 1mM and a maximal stimulation of 6- and 20-fold in cortical and hippocampal slices, respectively. Stimulation of PI hydrolysis by d-aspartate was largely reduced by pharmacological blockade of mGlu5 metabotropic glutamate receptors with 2-methyl-6-(phenylethynyl)pyridine. These findings suggest that d-aspartate behaves as an endogenous agonist of mGlu5 receptors during early postnatal life.

AB - The d-amino acid, d-aspartate, is abundant in the developing brain, yet its function is unknown. Addition of d-aspartate to hippocampal or cortical slices prepared from 8- to 9-day-old rats stimulated polyphosphoinositide (PI) hydrolysis to a slightly greater extent than l-glutamate. The action of d-aspartate was concentration-dependent with an apparent EC50 value of 1mM and a maximal stimulation of 6- and 20-fold in cortical and hippocampal slices, respectively. Stimulation of PI hydrolysis by d-aspartate was largely reduced by pharmacological blockade of mGlu5 metabotropic glutamate receptors with 2-methyl-6-(phenylethynyl)pyridine. These findings suggest that d-aspartate behaves as an endogenous agonist of mGlu5 receptors during early postnatal life.

KW - Cortical slices

KW - D-Aspartate

KW - Hippocampal slices

KW - Metabotropic glutamate receptors

KW - Polyphosphoinositide hydrolysis

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ER -

D-Aspartate activates mGlu receptors coupled to polyphosphoinositide hydrolysis in neonate rat brain slices

Abstract

Keywords

ASJC Scopus subject areas

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