Cytoplasmic proteases of rat liver parenchymal cells

George N. DeMartino

doi:10.1016/0006-291X(82)92145-3

Cytoplasmic proteases of rat liver parenchymal cells

George N. DeMartino

Research output: Contribution to journal › Article › peer-review

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Abstract

Soluble extracts of isolated rat liver parenchymal cells contained three proteases with alkaline pH optima. One protease was a high molecular weight (Mr = 500,000) enzyme which was stimulated by ATP. The other two proteases were totally dependent on calcium for activity and displayed different calcium concentration requirements. One was half-maximally activated by 150 μM Ca²⁺ while the other required only 10 μM Ca²⁺ for half-maximal activation.

Original language	English (US)
Pages (from-to)	1325-1330
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	108
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(82)92145-3
State	Published - Oct 15 1982

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Cytoplasmic proteases of rat liver parenchymal cells",

abstract = "Soluble extracts of isolated rat liver parenchymal cells contained three proteases with alkaline pH optima. One protease was a high molecular weight (Mr = 500,000) enzyme which was stimulated by ATP. The other two proteases were totally dependent on calcium for activity and displayed different calcium concentration requirements. One was half-maximally activated by 150 μM Ca2+ while the other required only 10 μM Ca2+ for half-maximal activation.",

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AB - Soluble extracts of isolated rat liver parenchymal cells contained three proteases with alkaline pH optima. One protease was a high molecular weight (Mr = 500,000) enzyme which was stimulated by ATP. The other two proteases were totally dependent on calcium for activity and displayed different calcium concentration requirements. One was half-maximally activated by 150 μM Ca2+ while the other required only 10 μM Ca2+ for half-maximal activation.

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Cytoplasmic proteases of rat liver parenchymal cells

Abstract

ASJC Scopus subject areas

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