Abstract
The histidine-containing phosphotransfer (HPt) domain at the C-terminus of the anaerobic sensor kinase ArcB has been cocrystallized with the chemotaxis response regulator CheY by a hanging-drop vapor-diffusion method. Crystals belong to space group P212121 with unit-cell dimensions a = 55.32, b = 76.29 and c = 83.89 Å, with one molecule in the crystallographic asymmetric unit. The crystals diffract to 2.7 Å resolution. This is the first crystallization of a protein-protein complex formed by a transmitter domain of sensor kinase and a receiver domain of response regulator in the two-component signal-transduction system.
Original language | English (US) |
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Pages (from-to) | 140-142 |
Number of pages | 3 |
Journal | Acta Crystallographica Section D: Biological Crystallography |
Volume | 54 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 1998 |
ASJC Scopus subject areas
- Structural Biology