Cotranslational biogenesis of NF-κB p50 by the 26S proteasome

Li Lin, George N. DeMartino, Warner C. Greene

Research output: Contribution to journalArticlepeer-review

260 Scopus citations


The NFKB1 gene encodes two functionally distinct proteins termed p50 and p105. p50 corresponds to the N terminus of p105 and with p65 (RelA) forms the prototypical NF-κB transcription factor complex. In contrast, p105 functions as a Rel-specific inhibitor (IκB) and has been proposed to be the precursor of p50. Our studies now demonstrate that p50 is generated by a unique cotranslational processing event involving the 26S proteasome, whereas cotranslational folding of sequences near the C terminus of p50 abrogates proteasome processing and leads to p105 production. These results indicate that p105 is not the precursor of p50 and reveal a novel mechanism of gene regulation that ensures the balanced production and independent function of the p50 and p105 proteins.

Original languageEnglish (US)
Pages (from-to)819-828
Number of pages10
Issue number6
StatePublished - Mar 20 1998

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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