Conversion of lytic to persistent alphavirus infection by the bcl-2 cellular oncogene

Beth Levine, Qi Huang, John T. Isaacs, John C. Reed, Diane E. Grifin, J. Marie Hardwick

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424 Scopus citations


Little is known about virus-host cell interactions that regulate the lytic potential of viruses during productive replication. Sindbis virus (SV), a single-stranded positive-sense RNA virus in the alphavirus genus (family Togaviridae), results in lytic infection in most vertebrate cell lines, but persistent productive infection in post-mitotic neurons1. The cellular oncogene bcl-2, which encodes an inner mitochondrial membrane protein of Mr 26,000 (ref. 2), blocks programmed cell death (apoptosis) in neurons3. We therefore investigated whether SV infection induces programmed cell death in non-neuronal cells, and if so, whether virus-induced programmed cell death can be blocked by transfection with bcl-2. We demonstrate that SV infection of baby hamster kidney (BHK-2), mouse neuroblastoma (N18), and rat prostatic adenocarcinoma (AT-3) cells results in programmed cell death, whereas SV infection of bcl-2-transfected AT-3 cells results in long-term persistent productive infection. Thus cellular bcl-2 oncogene expression plays a role in the establishment of persistent viral infection by blocking virus-induced programmed cell death.

Original languageEnglish (US)
Pages (from-to)739-742
Number of pages4
Issue number6414
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • General


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