Abstract
The present study evaluated the contribution of cytochrome P-450 ω- hydroxylase in modulating the reactivity of cremaster muscle arterioles in normotensive rats on high-salt (HS) and low-salt (LS) diet and in rats with reduced renal mass hypertension (RRM-HT). Changes in arteriolar diameter in response to ACh, sodium nitroprusside (SNP), ANG II, and elevated O2 were measured via television microscopy under control conditions and following cytochrome P-450 ω-hydroxylase inhibition with 17-octadecynoic acid (17- ODYA) or N-methylsulfonyl-12,12-dibromododec-11-enamide (DDMS). In normotensive rats on either LS or HS diet, resting tone was unaffected and arteriolar reactivity to ACh or SNP was minimally affected by cytochrome P- 450 ω-hydroxylase inhibition. In RRM-HT rats, cytochrome P-450 ω- hydroxylase inhibition reduced resting tone and significantly enhanced arteriolar dilation to ACh and SNP. Treatment with 17-ODYA or DDMS inhibited arteriolar constriction to ANG II and O2 in all the groups, although the degree of inhibition was greater in RRM-HT than in normotensive animals. These results suggest that metabolites of cytochrome P-450 ω-hydroxylase contribute to the altered reactivity of skeletal muscle arterioles to vasoconstrictor and vasodilator stimuli in RRM-HT.
Original language | English (US) |
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Pages (from-to) | H1517-H1526 |
Journal | American Journal of Physiology - Heart and Circulatory Physiology |
Volume | 278 |
Issue number | 5 47-5 |
DOIs | |
State | Published - May 2000 |
Keywords
- 17- octadecynoic acid
- 20-hydroxyeicosatetraenoic acid
- Acetylcholine
- Angiotensin II
- Microcirculation
- N-methylsulfonyl-12,12-dibromododec-11- enamide
- Oxygen
- Skeletal muscle
- Sodium nitroprusside
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
- Physiology (medical)