Contribution of Breastfeeding to False-Positive Saliva Polymerase Chain Reaction for Newborn Congenital Cytomegalovirus Screening

Shannon A. Ross; Marian G. Michaels; Amina Ahmed; April L. Palmer; Pablo J. Sánchez; David I. Bernstein; Kristina Feja; Audra Stewart; Suresh B. Boppana; Karen B. Fowler

doi:10.1093/infdis/jiy057

Contribution of Breastfeeding to False-Positive Saliva Polymerase Chain Reaction for Newborn Congenital Cytomegalovirus Screening

Shannon A. Ross, Marian G. Michaels, Amina Ahmed, April L. Palmer, Pablo J. Sánchez, David I. Bernstein, Kristina Feja, Audra Stewart, Suresh B. Boppana, Karen B. Fowler

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Real-time polymerase chain reaction (PCR) of saliva is highly sensitive for newborn congenital cytomegalovirus (CMV) screening. This study uses nationally published CMV seroprevalence and breastfeeding rates to estimate the contribution of CMV DNA in breast milk to false-positive saliva PCR results. The false-positive rates adjusted for breastfeeding ranged from 0.03% in white Hispanic persons to 0.14% in white non-Hispanic persons. Saliva CMV PCR for newborn screening is highly sensitive, and the low false-positive rates in this study suggest that saliva PCR results are unlikely to be significantly influenced by breastfeeding or other perinatal exposures.

Original language	English (US)
Pages (from-to)	1612-1615
Number of pages	4
Journal	Journal of Infectious Diseases
Volume	217
Issue number	10
DOIs	https://doi.org/10.1093/infdis/jiy057
State	Published - Apr 23 2018

Keywords

breastfeeding
cytomegalovirus
newborn
polymerase chain reaction
saliva
screening

ASJC Scopus subject areas

General Medicine

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10.1093/infdis/jiy057

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Ross, S. A., Michaels, M. G., Ahmed, A., Palmer, A. L., Sánchez, P. J., Bernstein, D. I., Feja, K., Stewart, A., Boppana, S. B., & Fowler, K. B. (2018). Contribution of Breastfeeding to False-Positive Saliva Polymerase Chain Reaction for Newborn Congenital Cytomegalovirus Screening. Journal of Infectious Diseases, 217(10), 1612-1615. https://doi.org/10.1093/infdis/jiy057

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abstract = "Real-time polymerase chain reaction (PCR) of saliva is highly sensitive for newborn congenital cytomegalovirus (CMV) screening. This study uses nationally published CMV seroprevalence and breastfeeding rates to estimate the contribution of CMV DNA in breast milk to false-positive saliva PCR results. The false-positive rates adjusted for breastfeeding ranged from 0.03% in white Hispanic persons to 0.14% in white non-Hispanic persons. Saliva CMV PCR for newborn screening is highly sensitive, and the low false-positive rates in this study suggest that saliva PCR results are unlikely to be significantly influenced by breastfeeding or other perinatal exposures.",

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AU - Ross, Shannon A.

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AU - Ahmed, Amina

AU - Palmer, April L.

AU - Sánchez, Pablo J.

AU - Bernstein, David I.

AU - Feja, Kristina

AU - Stewart, Audra

AU - Boppana, Suresh B.

AU - Fowler, Karen B.

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Contribution of Breastfeeding to False-Positive Saliva Polymerase Chain Reaction for Newborn Congenital Cytomegalovirus Screening

Abstract

Keywords

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