TY - JOUR
T1 - Concanavalin a increases the strength of baby hamster kidney cell attachment to substratum
AU - Grinnell, F.
PY - 1973/9/1
Y1 - 1973/9/1
N2 - The strength of attachment of normal and transformed baby hamster kidney cells was markedly increased when attached cells were treated with concanavalin A (Con A). The cells became less sensitive to detachment by physical shear or by treatment with trypsin or EDTA; however, their morphology, as observed by phase contrast microscopy, did not change. The effects of Con A were prevented by the simultaneous addition of either Dglucose or a-methyl-n-glucoside with the Con A. Also addition of these reagents to the attached cells after Con A treatment partially reversed the effects caused by Con A. Pretreatment of the culture flasks with Con A before cell attachment resulted in an increase in the strength of cell attachment to the culture flasks as compared to untreated controls.
AB - The strength of attachment of normal and transformed baby hamster kidney cells was markedly increased when attached cells were treated with concanavalin A (Con A). The cells became less sensitive to detachment by physical shear or by treatment with trypsin or EDTA; however, their morphology, as observed by phase contrast microscopy, did not change. The effects of Con A were prevented by the simultaneous addition of either Dglucose or a-methyl-n-glucoside with the Con A. Also addition of these reagents to the attached cells after Con A treatment partially reversed the effects caused by Con A. Pretreatment of the culture flasks with Con A before cell attachment resulted in an increase in the strength of cell attachment to the culture flasks as compared to untreated controls.
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U2 - 10.1083/jcb.58.3.602
DO - 10.1083/jcb.58.3.602
M3 - Article
C2 - 4201181
AN - SCOPUS:0015829718
SN - 0021-9525
VL - 58
SP - 602
EP - 607
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 3
ER -