Composition of adipose tissue and marrow fat in humans by 1H NMR at 7 Tesla

Research output: Contribution to journalArticlepeer-review

306 Scopus citations


Proton NMR spectroscopy at 7 Tesla (7T) was evaluated as a new method to quantify human fat composition noninvasively. In validation experiments, the composition of a known mixture of triolein, tristearin, and trilinolein agreed well with measurements by 1H NMR spectroscopy. Triglycerides in calf subcutaneous tissue and tibial bone marrow were examined in 20 healthy subjects by 1H spectroscopy. Ten well-resolved proton resonances from triglycerides were detected using stimulated echo acquisition mode sequence and small voxel (~0.1 ml), and T1 and T2 were measured. Triglyceride composition was not different between calf subcutaneous adipose tissue and tibial marrow for a given subject, and its variation among subjects, as a result of diet and genetic differences, fell in a narrow range. After correction for differential relaxation effects, the marrow fat composition was 29.1 ± 3.5% saturated, 46.4 ± 4.8% monounsaturated, and 24.5 ± 3.1% diunsaturated, compared with adipose fat composition, 27.1 ± 4.2% saturated, 49.6 ± 5.7% monounsaturated, and 23.4 ± 3.9% diunsaturated. Proton spectroscopy at 7T offers a simple, fast, noninvasive, and painless method for obtaining detailed information about lipid composition in humans, and the sensitivity and resolution of the method may facilitate longitudinal monitoring of changes in lipid composition in response to diet, exercise, and disease.

Original languageEnglish (US)
Pages (from-to)2055-2062
Number of pages8
JournalJournal of lipid research
Issue number9
StatePublished - Sep 2008


  • Bone marrow
  • Fatty acids
  • In vivo
  • Lipid composition
  • Metabolism
  • Musculoskeletal
  • Spectroscopy
  • Subcutaneous fat
  • Triglycerides

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology


Dive into the research topics of 'Composition of adipose tissue and marrow fat in humans by 1H NMR at 7 Tesla'. Together they form a unique fingerprint.

Cite this