Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice

James A. Lederer; Bernard H. Brownstein; M. Cecilia Lopez; Sandra MacMillan; Adam J. Delisle; Malcolm P. MacConmara; Mashkoor A. Choudhry; Wenzhong Xiao; Steven Lekousi; J. Perren Cobb; Henry V. Baker; John A. Mannick; Irshad H. Chaudry; Ulysses Balis; Paul Bankey; Timothy R. Billiar; Steven E. Calvano; David G. Camp; Joseph Cuschieri; Ronald W. Davis; Asit K. De; Constance Elson; Celeste C. Finnerty; Bradley Freeman; Richard L. Gamelli; Nicole S. Gibran; Brian G. Harbrecht; Douglas L. Hayden; Laura Hennessy; David N. Herndon; Jureta W. Horton; Marc G. Jeschke; Jeffrey Johnson; Matthew B. Klein; Stephen F. Lowry; Ronald V. Maier; Philip H. Mason; Grace P. McDonald-Smith; Carol L. Miller-Graziano; Michael N. Mindrinos; Joseph P. Minei; Lyle L. Moldawer; Ernest E. Moore; Avery B. Nathens; Grant E. O'Keefe; Laurence G. Rahme; Daniel G. Remick; David A. Schoenfeld; Michael B. Shapiro; Geoffrey M. Silver; Richard D. Smith; John Storey; Robert Tibshirani; Ronald G. Tompkins; Mehmet Toner; H. Shaw Warren; Michael A. West

doi:10.1152/physiolgenomics.00086.2007

Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice

James A. Lederer, Bernard H. Brownstein, M. Cecilia Lopez, Sandra MacMillan, Adam J. Delisle, Malcolm P. MacConmara, Mashkoor A. Choudhry, Wenzhong Xiao, Steven Lekousi, J. Perren Cobb, Henry V. Baker, John A. Mannick, Irshad H. Chaudry, Ulysses Balis, Paul Bankey, Timothy R. Billiar, Steven E. Calvano, David G. Camp, Joseph Cuschieri, Ronald W. DavisAsit K. De, Constance Elson, Celeste C. Finnerty, Bradley Freeman, Richard L. Gamelli, Nicole S. Gibran, Brian G. Harbrecht, Douglas L. Hayden, Laura Hennessy, David N. Herndon, Jureta W. Horton, Marc G. Jeschke, Jeffrey Johnson, Matthew B. Klein, Stephen F. Lowry, Ronald V. Maier, Philip H. Mason, Grace P. McDonald-Smith, Carol L. Miller-Graziano, Michael N. Mindrinos, Joseph P. Minei, Lyle L. Moldawer, Ernest E. Moore, Avery B. Nathens, Grant E. O'Keefe, Laurence G. Rahme, Daniel G. Remick, David A. Schoenfeld, Michael B. Shapiro, Geoffrey M. Silver, Richard D. Smith, John Storey, Robert Tibshirani, Ronald G. Tompkins, Mehmet Toner, H. Shaw Warren, Michael A. West

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A primary objective of the large collaborative project entitled "Inflammation and the Host Response to Injury" was to identify leukocyte genes that are differentially expressed after two different types of injury in mouse models and to test the hypothesis that both forms of injury would induce similar changes in gene expression. We report here the genes that are expressed in white blood cells (WBCs) and in splenocytes at 2 h, 1 day, 3 days, and 7 days after burn and sham injury or trauma-hemorrhage (T-H) and sham T-H. Affymetrix Mouse Genome 430 2.0 GeneChips were used to profile gene expression, and the results were analyzed by dCHIP, BRB Array Tools, and Ingenuity Pathway Analysis (IPA) software. We found that the highest number of genes differentially expressed following burn injury were at day 1 for both WBCs (4,989) and for splenocytes (4,715) and at day 1 for WBCs (1,167) and at day 3 for splenocytes (1,117) following T-H. The maximum overlap of genes that were expressed after both forms of injury were at day 1 in WBCs (136 genes) and at day 7 in splenocytes (433 genes). IPA revealed that the cell-to-cell signaling, cell death, immune response, antiapoptosis, and cell cycle control pathways were affected most significantly. In summary, this report provides a database of genes that are modulated in WBCs and splenocytes at sequential time points after burn or T-H in mice and reveals that relatively few leukocyte genes are expressed in common after these two forms of injury.

Original language	English (US)
Pages (from-to)	299-310
Number of pages	12
Journal	Physiological genomics
Volume	32
Issue number	3
DOIs	https://doi.org/10.1152/physiolgenomics.00086.2007
State	Published - Feb 19 2008

Keywords

Gene microarray
Gene microarray analysis
Immune response
Inflammation
Pathway analysis

ASJC Scopus subject areas

Physiology
Genetics

Access to Document

10.1152/physiolgenomics.00086.2007

Cite this

Lederer, J. A., Brownstein, B. H., Lopez, M. C., MacMillan, S., Delisle, A. J., MacConmara, M. P., Choudhry, M. A., Xiao, W., Lekousi, S., Cobb, J. P., Baker, H. V., Mannick, J. A., Chaudry, I. H., Balis, U., Bankey, P., Billiar, T. R., Calvano, S. E., Camp, D. G., Cuschieri, J., ... West, M. A. (2008). Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice. Physiological genomics, 32(3), 299-310. https://doi.org/10.1152/physiolgenomics.00086.2007

Lederer, JA, Brownstein, BH, Lopez, MC, MacMillan, S, Delisle, AJ, MacConmara, MP, Choudhry, MA, Xiao, W, Lekousi, S, Cobb, JP, Baker, HV, Mannick, JA, Chaudry, IH, Balis, U, Bankey, P, Billiar, TR, Calvano, SE, Camp, DG, Cuschieri, J, Davis, RW, De, AK, Elson, C, Finnerty, CC, Freeman, B, Gamelli, RL, Gibran, NS, Harbrecht, BG, Hayden, DL, Hennessy, L, Herndon, DN, Horton, JW, Jeschke, MG, Johnson, J, Klein, MB, Lowry, SF, Maier, RV, Mason, PH, McDonald-Smith, GP, Miller-Graziano, CL, Mindrinos, MN, Minei, JP, Moldawer, LL, Moore, EE, Nathens, AB, O'Keefe, GE, Rahme, LG, Remick, DG, Schoenfeld, DA, Shapiro, MB, Silver, GM, Smith, RD, Storey, J, Tibshirani, R, Tompkins, RG, Toner, M, Warren, HS & West, MA 2008, 'Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice', Physiological genomics, vol. 32, no. 3, pp. 299-310. https://doi.org/10.1152/physiolgenomics.00086.2007

@article{e03dba15efb047508eecdb26ef8a30f8,

title = "Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice",

abstract = "A primary objective of the large collaborative project entitled {"}Inflammation and the Host Response to Injury{"} was to identify leukocyte genes that are differentially expressed after two different types of injury in mouse models and to test the hypothesis that both forms of injury would induce similar changes in gene expression. We report here the genes that are expressed in white blood cells (WBCs) and in splenocytes at 2 h, 1 day, 3 days, and 7 days after burn and sham injury or trauma-hemorrhage (T-H) and sham T-H. Affymetrix Mouse Genome 430 2.0 GeneChips were used to profile gene expression, and the results were analyzed by dCHIP, BRB Array Tools, and Ingenuity Pathway Analysis (IPA) software. We found that the highest number of genes differentially expressed following burn injury were at day 1 for both WBCs (4,989) and for splenocytes (4,715) and at day 1 for WBCs (1,167) and at day 3 for splenocytes (1,117) following T-H. The maximum overlap of genes that were expressed after both forms of injury were at day 1 in WBCs (136 genes) and at day 7 in splenocytes (433 genes). IPA revealed that the cell-to-cell signaling, cell death, immune response, antiapoptosis, and cell cycle control pathways were affected most significantly. In summary, this report provides a database of genes that are modulated in WBCs and splenocytes at sequential time points after burn or T-H in mice and reveals that relatively few leukocyte genes are expressed in common after these two forms of injury.",

keywords = "Gene microarray, Gene microarray analysis, Immune response, Inflammation, Pathway analysis",

author = "Lederer, {James A.} and Brownstein, {Bernard H.} and Lopez, {M. Cecilia} and Sandra MacMillan and Delisle, {Adam J.} and MacConmara, {Malcolm P.} and Choudhry, {Mashkoor A.} and Wenzhong Xiao and Steven Lekousi and Cobb, {J. Perren} and Baker, {Henry V.} and Mannick, {John A.} and Chaudry, {Irshad H.} and Ulysses Balis and Paul Bankey and Billiar, {Timothy R.} and Calvano, {Steven E.} and Camp, {David G.} and Joseph Cuschieri and Davis, {Ronald W.} and De, {Asit K.} and Constance Elson and Finnerty, {Celeste C.} and Bradley Freeman and Gamelli, {Richard L.} and Gibran, {Nicole S.} and Harbrecht, {Brian G.} and Hayden, {Douglas L.} and Laura Hennessy and Herndon, {David N.} and Horton, {Jureta W.} and Jeschke, {Marc G.} and Jeffrey Johnson and Klein, {Matthew B.} and Lowry, {Stephen F.} and Maier, {Ronald V.} and Mason, {Philip H.} and McDonald-Smith, {Grace P.} and Miller-Graziano, {Carol L.} and Mindrinos, {Michael N.} and Minei, {Joseph P.} and Moldawer, {Lyle L.} and Moore, {Ernest E.} and Nathens, {Avery B.} and O'Keefe, {Grant E.} and Rahme, {Laurence G.} and Remick, {Daniel G.} and Schoenfeld, {David A.} and Shapiro, {Michael B.} and Silver, {Geoffrey M.} and Smith, {Richard D.} and John Storey and Robert Tibshirani and Tompkins, {Ronald G.} and Mehmet Toner and Warren, {H. Shaw} and West, {Michael A.}",

year = "2008",

month = feb,

day = "19",

doi = "10.1152/physiolgenomics.00086.2007",

language = "English (US)",

volume = "32",

pages = "299--310",

journal = "Physiological genomics",

issn = "1094-8341",

publisher = "American Physiological Society",

number = "3",

}

TY - JOUR

T1 - Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice

AU - Lederer, James A.

AU - Brownstein, Bernard H.

AU - Lopez, M. Cecilia

AU - MacMillan, Sandra

AU - Delisle, Adam J.

AU - MacConmara, Malcolm P.

AU - Choudhry, Mashkoor A.

AU - Xiao, Wenzhong

AU - Lekousi, Steven

AU - Cobb, J. Perren

AU - Baker, Henry V.

AU - Mannick, John A.

AU - Chaudry, Irshad H.

AU - Balis, Ulysses

AU - Bankey, Paul

AU - Billiar, Timothy R.

AU - Calvano, Steven E.

AU - Camp, David G.

AU - Cuschieri, Joseph

AU - Davis, Ronald W.

AU - De, Asit K.

AU - Elson, Constance

AU - Finnerty, Celeste C.

AU - Freeman, Bradley

AU - Gamelli, Richard L.

AU - Gibran, Nicole S.

AU - Harbrecht, Brian G.

AU - Hayden, Douglas L.

AU - Hennessy, Laura

AU - Herndon, David N.

AU - Horton, Jureta W.

AU - Jeschke, Marc G.

AU - Johnson, Jeffrey

AU - Klein, Matthew B.

AU - Lowry, Stephen F.

AU - Maier, Ronald V.

AU - Mason, Philip H.

AU - McDonald-Smith, Grace P.

AU - Miller-Graziano, Carol L.

AU - Mindrinos, Michael N.

AU - Minei, Joseph P.

AU - Moldawer, Lyle L.

AU - Moore, Ernest E.

AU - Nathens, Avery B.

AU - O'Keefe, Grant E.

AU - Rahme, Laurence G.

AU - Remick, Daniel G.

AU - Schoenfeld, David A.

AU - Shapiro, Michael B.

AU - Silver, Geoffrey M.

AU - Smith, Richard D.

AU - Storey, John

AU - Tibshirani, Robert

AU - Tompkins, Ronald G.

AU - Toner, Mehmet

AU - Warren, H. Shaw

AU - West, Michael A.

PY - 2008/2/19

Y1 - 2008/2/19

N2 - A primary objective of the large collaborative project entitled "Inflammation and the Host Response to Injury" was to identify leukocyte genes that are differentially expressed after two different types of injury in mouse models and to test the hypothesis that both forms of injury would induce similar changes in gene expression. We report here the genes that are expressed in white blood cells (WBCs) and in splenocytes at 2 h, 1 day, 3 days, and 7 days after burn and sham injury or trauma-hemorrhage (T-H) and sham T-H. Affymetrix Mouse Genome 430 2.0 GeneChips were used to profile gene expression, and the results were analyzed by dCHIP, BRB Array Tools, and Ingenuity Pathway Analysis (IPA) software. We found that the highest number of genes differentially expressed following burn injury were at day 1 for both WBCs (4,989) and for splenocytes (4,715) and at day 1 for WBCs (1,167) and at day 3 for splenocytes (1,117) following T-H. The maximum overlap of genes that were expressed after both forms of injury were at day 1 in WBCs (136 genes) and at day 7 in splenocytes (433 genes). IPA revealed that the cell-to-cell signaling, cell death, immune response, antiapoptosis, and cell cycle control pathways were affected most significantly. In summary, this report provides a database of genes that are modulated in WBCs and splenocytes at sequential time points after burn or T-H in mice and reveals that relatively few leukocyte genes are expressed in common after these two forms of injury.

AB - A primary objective of the large collaborative project entitled "Inflammation and the Host Response to Injury" was to identify leukocyte genes that are differentially expressed after two different types of injury in mouse models and to test the hypothesis that both forms of injury would induce similar changes in gene expression. We report here the genes that are expressed in white blood cells (WBCs) and in splenocytes at 2 h, 1 day, 3 days, and 7 days after burn and sham injury or trauma-hemorrhage (T-H) and sham T-H. Affymetrix Mouse Genome 430 2.0 GeneChips were used to profile gene expression, and the results were analyzed by dCHIP, BRB Array Tools, and Ingenuity Pathway Analysis (IPA) software. We found that the highest number of genes differentially expressed following burn injury were at day 1 for both WBCs (4,989) and for splenocytes (4,715) and at day 1 for WBCs (1,167) and at day 3 for splenocytes (1,117) following T-H. The maximum overlap of genes that were expressed after both forms of injury were at day 1 in WBCs (136 genes) and at day 7 in splenocytes (433 genes). IPA revealed that the cell-to-cell signaling, cell death, immune response, antiapoptosis, and cell cycle control pathways were affected most significantly. In summary, this report provides a database of genes that are modulated in WBCs and splenocytes at sequential time points after burn or T-H in mice and reveals that relatively few leukocyte genes are expressed in common after these two forms of injury.

KW - Gene microarray

KW - Gene microarray analysis

KW - Immune response

KW - Inflammation

KW - Pathway analysis

UR - http://www.scopus.com/inward/record.url?scp=40149110393&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40149110393&partnerID=8YFLogxK

U2 - 10.1152/physiolgenomics.00086.2007

DO - 10.1152/physiolgenomics.00086.2007

M3 - Article

C2 - 17986522

AN - SCOPUS:40149110393

SN - 1094-8341

VL - 32

SP - 299

EP - 310

JO - Physiological genomics

JF - Physiological genomics

IS - 3

ER -

Comparison of longitudinal leukocyte gene expression after burn injury or trauma-hemorrhage in mice

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this