TY - JOUR
T1 - Comparative analysis of microarray data identifies common responses to caloric restriction among mouse tissues
AU - Swindell, William R.
N1 - Funding Information:
This work was supported by NIA training grant AG000114 and the University of Michigan Department of Pathology. Helpful comments and suggestions were provided by two anonymous reviewers. The author thanks laboratories for providing microarray data to the Gene Expression Omnibus and ArrayExpress databases, as well as researchers who responded to requests for experimental data (Yoshikazu Higami, Yinghe Hu, Patricia L. Mote, Thomas A. Prolla, Steven R. Spindler, James M. Vann, Richard Weindruch and Pu Wu).
PY - 2008/3
Y1 - 2008/3
N2 - Caloric restriction has been extensively investigated as an intervention that both extends lifespan and delays age-related disease in mammals. In mice, much interest has centered on evaluating gene expression changes induced by caloric restriction (CR) in particular tissue types, but the overall systemic effect of CR among multiple tissues has been examined less extensively. This study presents a comparative analysis of microarray datasets that have collectively examined the effects of CR in 10 different tissue types (liver, heart, muscle, hypothalamus, hippocampus, white adipose tissue, colon, kidney, lung and cochlea). Using novel methods for comparative analysis of microarray data, detailed comparisons of the effects of CR among tissues are provided, and 28 genes for which expression response to CR is most shared among tissues are identified. These genes characterize common responses to CR, which consist of both activation and inhibition of stress-response pathways. With respect to liver tissue, transcriptional effects of CR exhibited surprisingly little overlap with those of aging, and a variable degree of overlap with the potential CR-mimetic drug resveratrol. These analyses shed light on the systemic transcriptional activity associated with CR diets, and also illustrate new approaches for comparative analysis of microarray datasets in the context of aging biology.
AB - Caloric restriction has been extensively investigated as an intervention that both extends lifespan and delays age-related disease in mammals. In mice, much interest has centered on evaluating gene expression changes induced by caloric restriction (CR) in particular tissue types, but the overall systemic effect of CR among multiple tissues has been examined less extensively. This study presents a comparative analysis of microarray datasets that have collectively examined the effects of CR in 10 different tissue types (liver, heart, muscle, hypothalamus, hippocampus, white adipose tissue, colon, kidney, lung and cochlea). Using novel methods for comparative analysis of microarray data, detailed comparisons of the effects of CR among tissues are provided, and 28 genes for which expression response to CR is most shared among tissues are identified. These genes characterize common responses to CR, which consist of both activation and inhibition of stress-response pathways. With respect to liver tissue, transcriptional effects of CR exhibited surprisingly little overlap with those of aging, and a variable degree of overlap with the potential CR-mimetic drug resveratrol. These analyses shed light on the systemic transcriptional activity associated with CR diets, and also illustrate new approaches for comparative analysis of microarray datasets in the context of aging biology.
KW - Aging
KW - Diet restriction
KW - Gene expression
KW - Longevity
KW - Microarray
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U2 - 10.1016/j.mad.2007.11.003
DO - 10.1016/j.mad.2007.11.003
M3 - Article
C2 - 18155270
AN - SCOPUS:39149086075
SN - 0047-6374
VL - 129
SP - 138
EP - 153
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
IS - 3
ER -