TY - JOUR
T1 - Common origin of the cerebellar dual somatotopic areas revealed by tracking embryonic purkinje cell clusters with birthdate tagging
AU - Tran-Anh, Khoa
AU - Zhang, Jingyun
AU - Nguyen-Minh, Viet Tuan
AU - Fujita, Hirofumi
AU - Hirata, Tatsumi
AU - Sugihara, Izumi
N1 - Funding Information:
Japan Society for the Promotion of Science (JSPS) [19K06919]; Japan Society for the Promotion of Science (JSPS) [20H03345] Acknowledgments: This study was supported by Grant-in-Aid for Scientific Research (KAKENHI) from the Japan Society for the Promotion of Science (19K06919 to I.S., 20H03345 to T.H.) and ROIS Challenging Exploratory Research Projects for the Future Grant to T.H. The authors thank Dr. Gideon Sarpong and Mr. Richard Nana Abankwah Owusu Mensah for proofreading the manuscript. The authors thank Dr. Yuichi Ono of KAN Research Institute Inc. for providing us with anti-Corl2 antibody.
Publisher Copyright:
© 2020 Tran-Anh et al.
PY - 2020
Y1 - 2020
N2 - One of the notable characteristics of the functional localization in the cerebellar cortex is the dual representation of the body (somatotopy) on its anterior-posterior axis. This somatotopy is conspicuous in the C1/C3 module, which is demarcated as the multiple zebrin-negative and weekly-positive stripes in dual paravermal areas in anterior and posterior lobules within the cerebellar compartments. In this report, we describe the early formation process of the cerebellar compartmentalization, particularly in the C1/C3 module. As developing PCs guide formation of the module-specific proper neuronal circuits in the cerebellum, we hypothesized that the rearrangement of embryonic Purkinje cell (PC) clusters shapes the adult cerebellar compartmentalization. By identifying PC clusters with immunostaining of marker molecules and genetical birthdate-tagging with Neurog2-CreER (G2A) mice, we clarified the three-dimensional spatial organization of the PC clusters and tracked the lineage relationships among the PC clusters from embryonic day 14.5 (E14.5) till E17.5. The number of recognized clusters increased from 9 at E14.5 to 37 at E17.5. Among E14.5 PC clusters, the c-l (central-lateral) cluster which lacked E10.5-born PCs divided into six c-l lineage clusters. They separately migrated underneath other clusters and positioned far apart mediolaterally as well as rostrocaudally by E17.5. They were eventually transformed mainly into multiple separate zebrin-negative and weakly-positive stripes, which together configured the adult C1/C3 module, in the anterior and posterior paravermal lobules. The results indi-cate that the spatial rearrangement of embryonic PC clusters is involved in forming the dual somatotopic areas in the adult mouse paravermal cerebellar cortex.
AB - One of the notable characteristics of the functional localization in the cerebellar cortex is the dual representation of the body (somatotopy) on its anterior-posterior axis. This somatotopy is conspicuous in the C1/C3 module, which is demarcated as the multiple zebrin-negative and weekly-positive stripes in dual paravermal areas in anterior and posterior lobules within the cerebellar compartments. In this report, we describe the early formation process of the cerebellar compartmentalization, particularly in the C1/C3 module. As developing PCs guide formation of the module-specific proper neuronal circuits in the cerebellum, we hypothesized that the rearrangement of embryonic Purkinje cell (PC) clusters shapes the adult cerebellar compartmentalization. By identifying PC clusters with immunostaining of marker molecules and genetical birthdate-tagging with Neurog2-CreER (G2A) mice, we clarified the three-dimensional spatial organization of the PC clusters and tracked the lineage relationships among the PC clusters from embryonic day 14.5 (E14.5) till E17.5. The number of recognized clusters increased from 9 at E14.5 to 37 at E17.5. Among E14.5 PC clusters, the c-l (central-lateral) cluster which lacked E10.5-born PCs divided into six c-l lineage clusters. They separately migrated underneath other clusters and positioned far apart mediolaterally as well as rostrocaudally by E17.5. They were eventually transformed mainly into multiple separate zebrin-negative and weakly-positive stripes, which together configured the adult C1/C3 module, in the anterior and posterior paravermal lobules. The results indi-cate that the spatial rearrangement of embryonic PC clusters is involved in forming the dual somatotopic areas in the adult mouse paravermal cerebellar cortex.
KW - Cerebellum
KW - Compartmentalization
KW - Purkinje cell
KW - Purkinje cell cluster
KW - Somatosensory
KW - Somatotopic representation
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U2 - 10.1523/ENEURO.0251-20.2020
DO - 10.1523/ENEURO.0251-20.2020
M3 - Article
C2 - 33055198
AN - SCOPUS:85098048421
SN - 2373-2822
VL - 7
SP - 1
EP - 23
JO - eNeuro
JF - eNeuro
IS - 6
ER -