Abstract
Objective: To study the combined effect of fluoride and arsenate on the expression of SD rat osteoblastic osteoclast differentiation factor (ODF) mRNA and osteoprotegerin (OPG) mRNA. Methods: Osteoblasts were obtained by enzymatic isolation from newborn SD rats. A factorial experiment was performed. Osteoblasts were exposed to NaF (0.5 mmolF/L, 4 molF/L) and NB 3AsH2 (12.5 μmolAs/L and 200 μmolAs/L) separately or F plus As and cultured for 48 h. The gene expression of osteoblastic ODF and OPG was observed by RT-PCR. Results: The expression of ODF mRNA increased in F0.5, F4 groups compared with control group and two groups of F0.5As200, F4As200 compared with As200 group, and decreased significantly in groups of F 4As125, F0.5As200, and F 4As200. The expression of OPG mRNA decreased in groups of F4, As200, F4As12.5, F 0.5As200, and F4As200. Conclusion: The joint effect of fluoride and arsenate on the gene expression of ODF is antagonistic, while the combined effect on the gene expression of OPG is synergistic. F4, F4As12.5, and F 0.5As200 promote bone resorption of rat osteoclasts, whereas F0.5As12.5 inhibits osteolytic effect of rat osteoclasts.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 375-379 |
| Number of pages | 5 |
| Journal | Biomedical and Environmental Sciences |
| Volume | 19 |
| Issue number | 5 |
| State | Published - Oct 1 2006 |
Keywords
- Arsenate
- Combined effect
- Fluoride
- ODF
- OPG
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Health, Toxicology and Mutagenesis