@article{7e6fcadd0e4d493ca175c2738dfb5474,
title = "Collagen Type 1 Accelerates Healing of Ruptured Fetal Membranes",
abstract = "Preterm premature rupture of membranes (pPROM) is a major cause of preterm birth. Recently, extracellular matrix-directed treatment is applied for wound healing. Here, we used a pregnant mouse model to test the efficacy of collagen type 1 gel for healing of the prematurely ruptured fetal membranes. Although injection of PBS into the ruptured fetal membranes resulted in 40% closure, injection of collagen type 1 improved closure rates to 90% within 72 h. Macrophages of the M2 wound healing phenotype were entrapped in the collagen layer. In primary human amnion mesenchymal cells, collagen type 1 gels activated collagen receptor discoidin domain receptor 2 (DDR2) to induce myosin light chain phosphorylation and migration of injured amnion mesenchymal cells. These findings define the mechanisms for matrix-directed therapeutics for pPROM.",
author = "Haruta Mogami and Kishore, {Annavarapu Hari} and Word, {R. Ann}",
note = "Funding Information: We thank Dr. Yucel Akgul (Department of Plastic Surgery, UT Southwestern) for assistance in immunofluorescent staining, Mr. Jesus{\textquoteright} Acevedo and Ms. Haolin Shi for pregnant mice preparation, the physicians and staff of Parkland Memorial Hospital, and Ms. Sylvia Wright for valuable assistance in tissue procurement. We also thank the shared resource cores in Microscopic Imaging, Molecular Pathology (Mr. John Shelton), Electron Microscopy (Dr. Anza Darehshouri and Ms. Phoebe Doss) at UTSWMC for technical support. This work was supported by March of Dimes Foundation Premature Research Initiation grants #21-FY13-35 and #21-FY15-138. The Tissue Acquisition Core supported by NIH P01HD087150 is gratefully acknowledged. Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41598-017-18787-9",
language = "English (US)",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}