Abstract
Co-immunoprecipitation (co-IP) of protein complexes from cell lysates is widely used to study protein-protein interactions. However, establishing robust co-IP assays often involves considerable optimization. Moreover, co-IP results are frequently presented in non-quantitative ways. This protocol presents an optimized co-IP workflow with an analysis based on semi-quantitative immunoblot densitometry to increase reliability and reproducibility. For complete details on the use and execution of this protocol, please refer to Burckhardt et al. (2021).
Original language | English (US) |
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Article number | 100644 |
Journal | STAR Protocols |
Volume | 2 |
Issue number | 3 |
DOIs | |
State | Published - Sep 17 2021 |
Keywords
- Cell Biology
- Gene Expression
- In Situ Hybridization
- Microscopy
- Model Organisms
- Molecular Biology
- Single Cell
- Single-molecule Assays
ASJC Scopus subject areas
- General Immunology and Microbiology
- General Biochemistry, Genetics and Molecular Biology
- General Neuroscience