Cloning multigene families with degenerate PCR primers

Thomas M. Wilkie, Melvin I. Simon

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Multigene families often exhibit regions of highly conserved amino acid sequence that are characteristic of a given family. Degenerate PCR primers that target these motifs will selectively amplify members within that multigene family. Members of the family that are expressed in a particular tissue can be amplified from cDNA. The PCR products can be easily cloned, screened, and sequenced over a precise region to rapidly identify novel genes. PCR with degenerate primers can also be used to screen the expression of these genes in various tissues and cell types.

Original languageEnglish (US)
Pages (from-to)32-41
Number of pages10
Issue number1
StatePublished - Feb 1991

ASJC Scopus subject areas

  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology


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